关键词: 蚊虫, 沃尔巴克氏体, wsp基因, 口岸

Abstract: Objective To investigate the infection status of Wolbachia and its phylogenetic relationships among common mosquitoes at Jiangsu ports, and to lay the foundation for mosquito control strategy based on Wolbachia. Methods During June to September in 2015, the light trap and carbon dioxide trap were used in 10 areas of Jiangsu ports(Suzhou, Changzhou, Wuxi, Nanjing, Nantong, Yangzhou, Taizhou, Lianyungang, Xuzhou, and Huaian), and DNA was extracted from the trapped mosquitoes, then Wolbachia wsp gene was amplified by PCR. All wsp genes were sequenced and phylogenetic analysis followed. Results We analyzed 5 species of common mosquitoes in Jiangsu ports, the total infection rate of Wolbachia was 26.80%, and the Culex pipiens complex infection rate(47.81%) was the highest, followed by Armigeres subalbatus(46.24%), Aedes albopictus(34.06%), and Cx. tritaeniorbynchus(1.25%). Anopheles sinensis were not infected. The mosquito Wolbachia wsp gene shared 10 haplotypes, and genetic distances between these haplotypes were 0.002-0.263, Hap-1 Hap-7, Hap-8, Hap-9, and Hap-10 were unique for the same mosquito species, while Hap-2, Hap-4, and Hap-5 in Jiangsu port mosquitoes appeared at high frequency, being shared in the 2-3 species. Among them, Hap-1 and Hap-2 belong to A group, while the rest belong to B group. Conclusion The common mosquitoes of Jiangsu ports have carried 2 species of Wolbachia, its intraspecific and interspecific distribution was highly variable. Through this investigation, the infection of Wolbachia at Jiangsu ports is preliminarily understood, the survey will serve the key of biological control based on the characteristics of Wolbachia.

Key words: Mosquito, Wolbachia, wsp gene, Port