中国媒介生物学及控制杂志 ›› 2018, Vol. 29 ›› Issue (1): 20-22.DOI: 10.11853/j.issn.1003.8280.2018.01.005

• 论著 • 上一篇    下一篇

我国室内常见蜚蠊的分子鉴定研究

陈红娜, 褚宏亮, 刘大鹏, 周明浩   

  1. 江苏省疾病预防控制中心消毒与媒介生物防制所, 南京 210009
  • 收稿日期:2017-09-01 出版日期:2018-02-20 发布日期:2018-02-20
  • 通讯作者: 周明浩,Email:zmh@jscdc.cn
  • 作者简介:陈红娜,女,主管技师,主要从事病媒生物防制研究工作,Email:418201387@qq.com
  • 基金资助:
    重大新发传染病综合防控科技示范工程(BE2015714);江苏省医学重点学科(流行病学)项目(ZDXKA2016008)

Molecular identification of common cockroaches in China

CHEN Hong-na, CHU Hong-liang, LIU Da-peng, ZHOU Ming-hao   

  1. Jiangsu Center for Disease Control and Prevention, Nanjing 210009, Jiangsu Province, China
  • Received:2017-09-01 Online:2018-02-20 Published:2018-02-20
  • Supported by:
    Supported by the Jiangsu Province Science and Technology Demonstration Project for Emerging Infectious Diseases Control and Prevention (No. BE2015714) and Jiangsu Province's Key Discipline (No. ZDXKA2016008)

摘要: 目的 分析我国室内15种常见蜚蠊的细胞色素C氧化酶亚基Ⅰ(COⅠ)基因序列,为建立蜚蠊分子鉴定方法奠定基础。方法 通过美国国立生物技术信息中心检索我国室内常见蜚蠊线粒体COⅠ基因序列,应用DNAStar和MEGA 6.0软件进行序列分析,探讨种间差异和遗传关系,利用Neighbor-Joining法构建系统进化树。结果 我国室内常见15种蜚蠊种平均进化分歧数为0.166,种间差异较大;系统进化树分析显示,同属蜚蠊COⅠ分子标记分类结果与传统形态分类学基本一致,且可信度均>91。结论 基于658 bp COⅠ基因序列,可对我国室内部分蜚蠊进行种下水平的分类鉴定,而该基因对于属或科以上水平的区分及少数近缘种的鉴定有所限制。

关键词: 蜚蠊, 分子鉴定, 细胞色素C氧化酶亚基Ⅰ

Abstract: Objective To provide basis for future molecular identification methods of cockroach species by analyzing gene sequences of cytochrome C oxidase subunitⅠ(COⅠ) among 15 cockroach species. Methods The mitochondrial COⅠ gene sequences of cockroaches were searched by NCBI and the sequence alignment was carried out by DNAStar software. An evolutionary divergence and a Neighbor-Joining tree were constructed using MEGA 6.0 software. Results The average interspecific genetic distances among 15 species of cockroaches in China was 0.166, and the genetic distances among species were significant. Phylogenetic tree analysis showed that COⅠ molecular markers were the same as traditional morphological classification, and the confidentiality more than 91. Conclusion The COⅠ gene can be effectively used to distinguish some species of domestic cockroaches. The value of 658 bp region of the mitochondrial COⅠ gene for identification of genus or subspecies is limited.

Key words: Cockroach, Molecular identification, Cytochrome C oxidase subunit Ⅰ

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