中国媒介生物学及控制杂志 ›› 2017, Vol. 28 ›› Issue (3): 248-250.DOI: 10.11853/j.issn.1003.8280.2017.03.013

• 论著 • 上一篇    下一篇

日本血蜱传播田鼠巴贝西虫的实验研究

吴嘉彤1, 张仪1, 朱丹1, 李兰花2   

  1. 1 中国疾病预防控制中心寄生虫病预防控制所, 卫生部寄生虫病原与媒介生物学重点实验室, 世界卫生组织热带病合作中心, 上海 200025;
    2 潍坊医学院公共卫生与管理学院, 山东 潍坊 261053
  • 收稿日期:2017-03-01 出版日期:2017-06-20 发布日期:2017-06-20
  • 通讯作者: 李兰花,Email:orchid8@sina.com
  • 作者简介:吴嘉彤,女,主管技师,主要从事媒介生物学研究,Email:wujiatong3@hotmail.com
  • 基金资助:
    国家卫生公益性行业科研专项重大专项(201202019);国家重点研发计划项目(2016YFC1202000,2016YFC1202001)

Experimental transmission of Babesia microti by Haemaphysalis japonica

WU Jia-tong1, ZHANG Yi1, ZHU Dan1, LI Lan-hua2   

  1. 1 National Institute of Parasitic Disease, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology, Ministry of Health, WHO Collaborating Center for Tropical Diseases, Shanghai 200025, China;
    2 School of Public Health, Weifang Medical College
  • Received:2017-03-01 Online:2017-06-20 Published:2017-06-20
  • Supported by:
    Supported by the Special Fund for Health Research in the Public Interest China(No. 201202019)and National Key Research and Development Program of China(No. 2016YFC1202000, 2016YFC1202001)

摘要: 目的 研究日本血蜱传播田鼠巴贝西虫的能力。方法 用日本血蜱幼蜱、若蜱和成蜱叮咬田鼠巴贝西虫阳性BALB/c小鼠,收集饱血蜱,待孵化为下一个龄期或产卵后,利用巢氏PCR法检测幼蜱的感染情况;再用感染性成蜱叮咬阴性NOD/SCID小鼠,定期采集鼠血进行检测,以观察小鼠感染情况。结果 幼蜱叮咬阳性小鼠发育为若蜱后,未检出田鼠巴贝西虫感染;若蜱叮咬阳性小鼠发育为成蜱后,8只雌性成蜱检出田鼠巴贝西虫感染,而雄性成蜱未检出田鼠巴贝西虫感染;成蜱叮咬阳性小鼠后,子代幼蜱未检出田鼠巴贝西虫感染。而12只阴性NOD/SCID小鼠被阳性成蜱叮咬后,有2只小鼠感染。结论 日本血蜱仅在若蜱-成蜱阶段可经期传播田鼠巴贝西虫,幼蜱-若蜱阶段不能经期传播田鼠巴贝西虫,亦不能经卵传播田鼠巴贝西虫。

关键词: 田鼠巴贝西虫, 日本血蜱, 经期传播, 经卵传播

Abstract: Objective To identify the transmission capacity of Babesia microti through Haemaphysalis japonica. Methods The larvae, nymphs, and adults of H. japonica were fed on BALB/c mice infected with B. microti. The engorged ticks were collected and maintained to the next stage. Ticks of the next stage or the offspring larvae were detected by nested PCR. NOD/SCID mice were then infested by adult ticks molting from nymphs that ingested the blood of infective mice, and the blood samples of NOD/SCID mice were then analyzed by PCR. Results None of the nymphs developed from larvae that fed on positive mice tested positive. Among adult ticks developed from nymphs that were exposed to positive mice, 40% of the females and none of the males tested positive. Among the 12 mice infested by infective adults, B. microti was detected in two NOD/SCID. None of the offspring larvae developed from adult ticks that fed on infected mice tested positive. Conclusion Experimental transstadial transmission of H. japonica for B. microti was proved in the nymph to adult route, but neither in the larvae to nymph routes nor transovarial transmission.

Key words: Babesia microti, Haemaphysalis japonica, Transstadial transmission, Transovarial transmission

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