Objective To survey the species distribution, community composition, and the blood-sucking rate of the main hematophagous midges of Suiyang county of Guizhou province, China. Methods The light (photocatalytic UV trap lamp) trap was used to capture blood sucking midges in different livestock sheds, which were then classified and identified. Results We obtained 31 167 blood sucking midges in Suiyang belonging to 2 genera 18 species, of which 15 species belonged to Culicoides, 3 species belonged to Lasiohelea. There were 10 new recorded species in Guizhou province, C. gymnopterus, C. kinabaluensis, C. mcdonaldi, C. nipponensis, C. orientalis, C. qianweiensis, C. tentorius, La. lushana, La. relicta, and La. ripa. The predominant species was C. orientalis (72.08%). The main species composition of the blood sucking midges varied among different livestock sheds. The total blood sucking rate of three main species was 40.12%, in which C. punctatus had the highest (56.41%) and C. orientalis had the lowest (36.88%) blood sucking rate. The three main hematophagous midge species had a different blood sucking rate in different livestock sheds. Conclusion The hematophagous midge species and blood sucking rate varied in different livestock sheds in Suiyang, which provides a basic data for the prevention and control of blood sucking midges and the species diversity research in Guizhou province.