中国媒介生物学及控制杂志 ›› 2017, Vol. 28 ›› Issue (2): 117-119.DOI: 10.11853/j.issn.1003.8280.2017.02.005

• 论著 • 上一篇    下一篇

中国-哈萨克斯坦边境阿拉山口口岸地区凶小库蚊中首次检测到沃尔巴克氏体

尹小平1, 王安东1,2, 田延河1, 巴特1, 张江国1, 梁臻1   

  1. 1 阿拉山口出入境检验检疫局, 新疆 阿拉山口 833418;
    2 新疆石河子大学动物科技学院, 新疆 石河子 832000
  • 收稿日期:2016-11-21 出版日期:2017-04-20 发布日期:2017-04-20
  • 作者简介:尹小平,男,副研究员,从事检验检疫及媒介生物监测工作,Email:yxpciq@163.com
  • 基金资助:

    国家质检总局科技计划项目(2016IK264)

First detection of Wolbachia in Culex modestus at Alataw port, China-Kazakhstan border

YIN Xiao-ping1, WANG An-dong1,2, TIAN Yan-he1, BA Te1, ZHANG Jiang-guo1, LIANG Zhen1   

  1. 1 Alashankou Entry-Exit Inspection and Quarantine Bureau, Alashankou 833418, Xinjiang Uygur Autonomous Region, China;
    2 College of Animal Science and Technology, Shihezi University
  • Received:2016-11-21 Online:2017-04-20 Published:2017-04-20
  • Supported by:

    Supported by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China Scientific Research Subject(No. 2016IK264)

摘要:

目的 调查中国-哈萨克斯坦(中哈)边境阿拉山口口岸地区优势蚊种凶小库蚊携带沃尔巴克氏体情况及其基因型,为防控蚊媒传播疾病提供依据。方法 2015年4-10月,在中哈边境一区、二区、阿拉山口艾比湖湿地自然保护区,利用诱蚊灯和挥网法采集蚊虫,PCR扩增沃尔巴克氏体wsp基因,测序后进行Blast分析,利用Mega 6.0软件构建分子遗传进化树。结果 凶小库蚊感染沃尔巴克氏体的阳性率为3.33%(2/60);wsp基因遗传进化树显示,沃尔巴克氏体wsp基因序列属于A(wAlbA)和B(wAlbB)2个超级基因组。结论 中哈边境阿拉山口口岸地区首次检测到凶小库蚊携带沃尔巴克氏体,其基因型为wAlbA和wAlbB型,为应用沃尔巴克氏体通过种群替换阻断蚊媒疾病的传播等防控技术提供了基础资料。

关键词: 沃尔巴克氏体, 凶小库蚊, 聚合酶链式反应, 中国-哈萨克斯坦边境

Abstract:

Objective To investigate the infection and genotype of Wolbachia from Culex modestus at Alataw port, China-Kazakhstan border. Methods Sweeping Netting and Light Trapping were utilized to collect adult mosquitoes at the Yiqu, Erqu and Aibi Lake, wsp genes of Wolbachia were detected by polymerase chain reaction (PCR), the sequence analysis by Blast and phylogenetic analysis by Mega 6.0. Results The positive rate of wsp gene of Wolbachia was 3.33% (2/60) at Alataw port. The wsp haplotype sequences were divided in Supergroup A (wAlbA) and B (wAlbB) by phylogenetic analysis. Conclusion The wAlbA and wAlbB Wolbachia exists in Cx. modestusat Alataw China-Kazakhstan port.

Key words: Wolbachia, Culex modestus, Polymerase chain reaction, China-Kazakhstan border

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