Identification and distribution of Apodemus species with DNA barcoding in China
LIU Rong-rong1, GE De-yan2, LU Liang1, XIA Lin2, LIU Qi-yong1, YANG Qi-sen2
1 State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
2 Institute of Zoology, Chinese Academy of Sciences
Objective To investigate the taxonomy and distribution of Apodemus species in China, and to provide supportive data for prevention and control of rodent borne disease. Methods DNA barcoding was employed to identify Apodemus samples collected from 22 provincial areas in China. Genomic DNA was extracted from tissue samples for PCR amplification of the mitochondrial cytochrome C oxidase subunitⅠ (COⅠ) gene, followed by sequencing. The obtained sequences were compared with the related sequences in GenBank. Then a phylogenetic tree was constructed. Results COⅠ gene fragment were amplified from 119 Apodemus samples, and they were sorted into eight branches through phylogenetic reconstruction. Compared with the data in the GenBank, the collected samples were identified as eight independent species: Ap. agrarius, Ap. chevrieri, Ap. peninsulae, Ap. latronum, Ap. ilex, Ap. draco, Ap. uralensis and an undefined species. Conclusion The accurate distribution of Apodemus species in China can be determined by effective identification of samples through DNA barcoding. In addition, the results revised the Apodemus species distribution based on the morphological identification.
刘蓉蓉, 葛德燕, 鲁亮, 夏琳, 刘起勇, 杨奇森. 中国姬鼠属种类的DNA条形码鉴定及其分布[J]. 中国媒介生物学及控制杂志, 2017, 28(2): 97-103.
LIU Rong-rong, GE De-yan, LU Liang, XIA Lin, LIU Qi-yong, YANG Qi-sen. Identification and distribution of Apodemus species with DNA barcoding in China. Chines Journal of Vector Biology and Control, 2017, 28(2): 97-103.
2017, Vol. 28 
