Research of the cytochrome C oxidase subunitⅠ gene polymorphisms for Rattus norvegicus in Jiangsu port
HU Shuang-shuang1, WU Bing-yao2, HE Jiang2, LU Yong-chang3, YANG Qing-gui2, CHEN Feng4, SUN Li-xin2
1 School of Medicine, Anhui University of Science and Technology, Huainan 232001, Anhui Province, China;
2 Jiangsu International Travel Healthcare Center;
3 Jiangsu Entry-Exit Inspection and Quarantine Bureau;
4 Nantong Entry-Exit Inspection and Quarantine Bureau
Objective The cytochrome C oxidase subunitⅠ(COⅠ) gene polymorphisms for Rattus norvegicus, was studied to provide support for the species identification of rodents from frontier port. Methods The COⅠ gene of R. norvegicus was amplified, sequenced, aligned and compared with the information on GenBank to analyze genetic polymorphism and create phylogenetic tree for R. norvegicus. Results Forty-eight COⅠ gene sequences were obtained from R. norvegicus. In this research, 605 loci were measured in each sequence, there were 587 conservative loci and 18 mutation loci, the nucleotide differences number was (2.510 000±0.000 640) on average. All of R. norvegicus in Jiangsu port background only shared one haploid type, other R. norvegicus' nucleotide polymorphism was rich. Conclusion There are species differences of the R. norvegicus in different areas, this kind of difference is related to the geographical origin.
胡双双, 吴炳耀, 何江, 陆永昌, 杨庆贵, 陈峰, 孙立新. 江苏口岸褐家鼠线粒体DNA细胞色素C氧化酶亚基Ⅰ基因的遗传多态性研究[J]. 中国媒介生物学及控制杂志, 2017, 28(1): 31-34.
HU Shuang-shuang, WU Bing-yao, HE Jiang, LU Yong-chang, YANG Qing-gui, CHEN Feng, SUN Li-xin. Research of the cytochrome C oxidase subunitⅠ gene polymorphisms for Rattus norvegicus in Jiangsu port. Chines Journal of Vector Biology and Control, 2017, 28(1): 31-34.
2017, Vol. 28 
