关键词: 褐小家鼠, 国内未见分布种, DNA条形码

Abstract:

Objective DNA barcoding techniques were employed to identify the unknown mouse species intercepted from the dry cassava imported from Cambodia at Zhongshan port, whose morphology was damaged too badly to be identified. Methods Genomic DNA was extracted from the intercepted mouse. Target fragments were amplified with the universal primers LCO1490 and HCO2198 for the metazoan mitochondrial cytochrome oxidaseⅠ (COⅠ), PCR products were sequenced. The sequences were blasted in the NCBI. Results The sequence homology of the DNA barcode fragment between these two samples were 99.8%, and was more than 98.5% identical to the sequence of Mus cervicolor published in NCBI. Mus cervicolor is never recorded in China before, and it was the first time to intercept this species at the Chinese port according to the references. Conclusion The intercepted mouse was M. cervicolor identified with DNA barcodes, a non-recorded species in China. DNA barcoding techniques could break the restrictions of traditional species identification depending on undamaged morphological characteristics, can improve the ability to identify the exotic species, overcome the difficult in lack of identification references and experts.

Key words: Mus cervicolor, Non-distributed species in China, DNA barcoding