关键词: 西尼罗病毒, 蚊媒, 反转录-聚合酶链反应

Abstract:

Objective To study the community structure, dynamic and West Nile virus (WNV) carried of mosquito vectors in Guangdong, and provide scientific evidence for further development of control and prevention strategies. Methods Mosquito density was monitored by Light-trap from May 2013 to April 2014. One-step reverse-transcript PCR was applied. Results The average density of mosquito was 11.80/light. The seasonal peak of density was from May to July. Of all habitats the density in the barn was the highest. Culex pipiens quinquefasciatus accounts for 89.90%, the largest proportion of mosquitoes. No positive was found by RT-PCR. Conclusion Culex pipiens quinquefasciatus was the predominant species, the monitoring data could provide scientific basis for the control strategies and warning and forecasting of WNV, but the vectors species of West Nile fever in Guangdong province and its spread risk needs to be further studied.

Key words: West Nile virus, Mosquito vector, Reverse transcription-polymerase chain reaction