深圳市白纹伊蚊细胞色素C氧化酶亚基Ⅰ基因多态性分析

doi:10.11853/j.issn.1003.4692.2015.04.002

中国媒介生物学及控制杂志 ›› 2015, Vol. 26 ›› Issue (4): 337-340.DOI: 10.11853/j.issn.1003.4692.2015.04.002

深圳市白纹伊蚊细胞色素C氧化酶亚基Ⅰ基因多态性分析

阳帆, 张仁利, 刘阳, 张韶华, 黄达娜, 吴春利, 李玥, 唐屹君

深圳市疾病预防控制中心, 重大传染病监控重点实验室, 广东深圳518055

收稿日期:2015-05-01 出版日期:2015-08-20 发布日期:2015-08-20
作者简介:阳帆,女,硕士,副主任技师,主要从事虫媒传染病预防控制相关研究,Email: sailing_728@163.com
基金资助:
广东省医学科研基金(B2013356)

Genetic polymorphism analysis on mtDNA-COⅠ of Aedes albopictus in Shenzhen, China

YANG Fan, ZHANG Ren-li, LIU Yang, ZHANG Shao-hua, HUANG Da-na, WU Chun-li, LI Yue, TANG Yi-jun

Key Laboratory on Major Infectious Disease Control, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, Guangdong Province, China

Received:2015-05-01 Online:2015-08-20 Published:2015-08-20
Supported by:
Supported by the Medical Research Foundation in Guangdong Province(No. B2013356)

摘要/Abstract

摘要：

目的研究深圳市白纹伊蚊线粒体细胞色素氧化酶亚基Ⅰ(mtDNA-COⅠ)基因多态性,探讨其遗传特征。方法在深圳市不同区域现场采集8个点的白纹伊蚊幼虫,实验室饲养,收集羽化成蚊,75%乙醇-20 ℃保存。单蚊抽提白纹伊蚊基因组DNA,PCR特异性扩增mtDNA-COⅠ基因,并从网上下载部分白纹伊蚊mtDNA-COⅠ进行序列分析和比对,用生物信息系统进行mtDNA-COⅠ基因多态性分析。结果 PCR特异扩增后获得白纹伊蚊mtDNA-COⅠ基因1433 bp,序列分析深圳市8个不同地理区域的白纹伊蚊mtDNA-COⅠ的同一性为99%,其mtDNA-COⅠ基因单核苷酸存在转换、颠换,共出现24个变异位点,变异率为1.67%。而同一地理环境的5只白纹伊蚊mtDNA-COⅠ基因序列无差异。结论深圳地区不同采样点白纹伊蚊在mtDNA-COⅠ基因有高度的保守性,不同个体也表现了单核苷酸的多态性,其形成原因有待进一步研究。

关键词: 白纹伊蚊, 遗传特征, 线粒体细胞色素氧化酶亚基Ⅰ, 基因多态性

Abstract:

Objective To study the characteristics of mitochondrial DNA cytochrome oxidase subunitⅠ (mtDNA-COⅠ) gene and the spatial population genetic diversity of Aedes albopictus in Shenzhen. Methods Larvae of Ae. albopictus were collected from eight investigation sites in Longgang, Guangming, Futian,Dapeng, Luohu, Longhua, Nanshan and Baoan district, and reared in the laboratory, and the resulted adults were preserved in 75% alcohol under -20 ℃. Single mosquito genomic DNA was extracted from Ae. albopictus of different populations, and mtDNA-COⅠ gene were specifically amplified by PCR. The amplified mtDNA-COⅠ gene sequences in different geographic strains were analyzed and compared with the others in the GenBank, and genetic polymorphism analysis of them was conducted by bio-information system. Results The obtained COⅠ sequences of Ae. albopictus of eight different isolates from Shenzhen showed 99% homogeny with 1433 bp in length. As there were transition and transversion in the mononucleotide of mtDNA-COⅠ gene, Ae. albopictus from different geographical locations in Shenzhen showed gene polymorphism in sequence analysis on mtDNA-COⅠ with 24 mutation sites and 1.67% mutation rate. However, there was no difference in gene sequence of mtDNA-COⅠ in the five mosquitoes of Ae. albopictus from the same geographical location. Conclusion The mtDNA-COⅠ gene of Ae. albopictus from different collection sites of Shenzhen have high homogeny, but single nuclear polymorphism(SNP) was also observed among different Ae. albopictus populations. The SNP forming reason needs to be further studied.

Key words: Aedes albopictus, Genetic characteristics, Mitochondrial DNA cytochrome oxidase subunit Ⅰ, Genetic polymorphism

中图分类号:

R384.1

阳帆, 张仁利, 刘阳, 张韶华, 黄达娜, 吴春利, 李玥, 唐屹君. 深圳市白纹伊蚊细胞色素C氧化酶亚基Ⅰ基因多态性分析[J]. 中国媒介生物学及控制杂志, 2015, 26(4): 337-340.

YANG Fan, ZHANG Ren-li, LIU Yang, ZHANG Shao-hua, HUANG Da-na, WU Chun-li, LI Yue, TANG Yi-jun. Genetic polymorphism analysis on mtDNA-COⅠ of Aedes albopictus in Shenzhen, China[J]. Chines Journal of Vector Biology and Control, 2015, 26(4): 337-340.

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深圳市白纹伊蚊细胞色素C氧化酶亚基Ⅰ基因多态性分析

Genetic polymorphism analysis on mtDNA-COⅠ of Aedes albopictus in Shenzhen, China

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