中国媒介生物学及控制杂志 ›› 2015, Vol. 26 ›› Issue (1): 84-85.DOI: 10.11853/j.issn.1003.4692.2015.01.023

• 调查研究 • 上一篇    下一篇

豚鼠感染鼠疫菌脏器的病理改变

赵忠智1, 于守鸿1, 张爱萍1, 党占翠2, 王梅1, 郑谊1, 熊浩明1, 于志敏3, 赵海龙2, 王祖郧1   

  1. 1. 青海省地方病预防控制所鼠疫预防控制科, 西宁811602;
    2. 青海大学医学院;
    3. 青海大学附属医院
  • 收稿日期:2014-08-25 出版日期:2015-02-20 发布日期:2015-02-20
  • 通讯作者: 王祖郧,Email: wangzuyun@163.com
  • 作者简介:赵忠智,男,主管医师,主要从事鼠疫病原学检验,Email: zhzhzh1026@sohu.com

Pathological changes in solid viscera of Guinea pigs infected with Yersinia pestis

ZHAO Zhong-zhi1, YU Shou-hong1, ZHANG Ai-ping1, DANG Zhan-cui2, WANG Mei1, ZHENG Yi1, XIONG Hao-ming1, YU Zhi-min3, ZHAO Hai-long2, WANG Zu-yun1   

  1. 1. Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, Qinghai Province, China;
    2. Qinghai University Medical College;
    3. Qinghai University Affiliated Hospital
  • Received:2014-08-25 Online:2015-02-20 Published:2015-02-20

摘要:

目的 探讨豚鼠感染鼠疫菌主要实质性脏器的病理改变。方法 强毒鼠疫菌25 cfu/ml感染成体实验豚鼠鼠蹊部皮下。病死豚鼠立即取材,未病死豚鼠14 d分别处死并取材。取肝、脾、肺、肾及心等实质性脏器分离培养鼠疫菌,并制作病理切片观察病理改变。结果 感染鼠疫菌的实验豚鼠肝、脾、肺、肾及心脏均分离出鼠疫菌;其组织巨检均有明显病理变化,镜检除心脏外均有明显的病理变化,呈炎性细胞增多,充血、出血、部分坏死等急性炎症变化。结论 强毒鼠疫菌引起实验豚鼠急性炎症反应,豚鼠肝、脾、肺、肾、心等实质性脏器的病理变化显著。

关键词: 鼠疫耶尔森菌, 组织病理学

Abstract:

Objective To investigate pathological changes in the major solid viscera of guinea pigs infected with the plague bacillus Yersinia pestis. Methods Adult guinea pigs were infected with a virulent Y. pestis strain (25 cfu/ml) through the groin subcutaneously. After infection, tissue specimens were immediately taken from dead animals, and the surviving ones were executed 14 d later. Specimens of solid viscera, including the liver, spleen, lung, kidney, and heart, were used for isolation of Y. pestis, and section specimens were prepared to observe associated pathological changes. Results Yersinia pestis was isolated from the liver, spleen, lung, kidney, and heart of the experimental animals after infection. Tissue autopsy revealed significant pathological changes in all solid viscera, and microscopic examination showed obvious pathological changes in the major organs other than the heart. There were acute inflammatory changes such as inflammatory cell increase, hyperemia, hemorrhage, and partial necrosis. Conclusion Virulent Y. pestis can cause acute inflammation in guinea pigs and result in significant pathological changes in solid viscera of the animals, including the liver, spleen, lung, kidney, and heart.

Key words: Yersinia pestis, Histopathology

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