关键词: 河北省坝上地区, 夜行鼠, DNA条形码, 细胞色素C氧化酶亚基Ⅰ基因

Abstract:

Objective To identify nocturnal rodent species in the Bashang area of Hebei province, China, using DNA barcoding. Methods Liver tissue samples of nocturnal rodents were collected from the study area. The whole mice were also preserved and dissected. Genomic DNA was extracted for PCR amplification of the mitochondrial cytochrome c oxidase subunitⅠ (COⅠ) gene with consensus primers, followed by sequencing. Blast comparisons were performed between the obtained sequencing data and the DNA bar codes of other rodent species in the GenBank. A molecular evolutionary tree was built. Results All the 36 samples produced specific COⅠ gene bands by PCR amplification. The result of molecular evolutionary tree was slightly different from that of morphological identification. Skull identification revealed that the molecular evolutionary tree was correct, and the result of morphological identification was corrected. High-homology genes were not found in the GenBank for two nocturnal rodents. Since their skulls were seriously damaged, we temporally could not determine the species of these two individuals. Conclusion DNA barcoding can be used to effectively identify rodent species.

Key words: Hebeiprovince,Bashangarea, Nocturnalrodent, DNAbarcoding, Mitochondrialgene, CytochromecoxidasesubunitⅠ