拉萨市2009－2013年蚊虫种类构成及密度动态研究

doi:10.11853/j.issn.1003.4692.2014.03.002

中国媒介生物学及控制杂志 ›› 2014, Vol. 25 ›› Issue (3): 200-204.DOI: 10.11853/j.issn.1003.4692.2014.03.002

拉萨市2009－2013年蚊虫种类构成及密度动态研究

刘小波¹, 次仁顿珠², 郭玉红¹, 彭措次仁², 白莉¹, 桑少伟¹, 白玛次旺², 谷少华¹, 达珍², 陈斌¹, 周琳¹, 万方君¹, 胡亚萍¹, 徐俊芳³, 王君¹, 达瓦², 李贵昌¹, 西绕若登², 刘起勇¹

1 中国疾病预防控制中心传染病预防控制所, 传染病预防控制国家重点实验室, 中国疾病预防控制中心传染病监测预警重点实验室, 感染性疾病诊治协同创新中心, 世界卫生组织媒介生物监测与管理合作中心, 北京 102206;
2 西藏自治区疾病预防控制中心, 拉萨 850000;
3 山东大学卫生管理与政策研究中心

收稿日期:2014-02-24 出版日期:2014-06-20 发布日期:2014-06-20
通讯作者: 刘起勇, Email: liuqiyong@icdc.cn
作者简介:刘小波, 男, 博士, 助理研究员, 主要从事媒介生物监测、风险评估及预警研究。次仁顿珠, 男, 副主任医师, 主要从事传染病学研究。
基金资助:
国家重大科学研究计划(2012CB955504); 国家“十二五”科技重大专项课题(2012ZX10004219-002)

A survey of species composition and population dynamics of mosquitoesin Lhasa, Tibet, China from 2009 to 2013

LIU Xiao-bo¹, Cirendunzhu², GUO Yu-hong¹, Pengcuociren², BAI Li¹, SANG Shao-wei¹, Baimaciwang², GU Shao-hua¹, Dazhen², CHEN Bin¹, ZHOU Lin¹, WAN Fang-jun¹, HU Ya-ping¹, XU Jun-fang³, WANG Jun¹, Dawa², LI Gui-chang¹, Xiraoruodeng², LIU Qi-yong¹

1 State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, China CDC Key Laboratory of Surveillance and Early?Warning on Infectious Disease, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, WHO Collaborating Centrefor Vector Surveillance and Management, Beijing 102206, China;
2 Tibet Center for Disease Control and Prevention, Lhasa 850000, Tibet, China;
3 Center for Health Management and Policy, Shandong University

Received:2014-02-24 Online:2014-06-20 Published:2014-06-20
Contact: LIU Qi-yong, Email: liuqiyong@icdc.cn

摘要/Abstract

摘要：

目的掌握拉萨市蚊虫种类构成及密度动态, 为该市媒介蚊虫及蚊媒传染病监测及防控提供基础数据。方法在拉萨市城区不同方位选取调查点, 以诱蚊灯法、人帐诱法及人工小时法捕获蚊类, 用形态学和多重PCR方法进行蚊种鉴定。结果拉萨市蚊类包括尖音库蚊复合组蚊虫亚种和骚扰阿蚊, 优势蚊种为尖音库蚊复合组蚊虫亚种。2009、2012年人帐诱法总蚊密度分别为19.57和3.06只/(帐·h), 不同年份不同调查点蚊密度差异无统计学意义(F1, 1＝0.934, P＞0.05)。2009、2012年人工小时法总蚊密度分别为62.10和7.90只/人工小时, 不同年份不同调查点蚊密度差异无统计学意义(F1, 1＝0.874, P＞0.05)。2012、2013年诱蚊灯法总蚊密度分别为1.47和0.20只/(灯·h)。不同年份不同调查点居民区蚊密度差异亦无统计学意义(F1, 3＝1.503, P＞0.05)。2012年诱蚊灯法捕获的105只蚊虫中, 经多重PCR鉴定, 36 只属尖音库蚊复合组蚊虫亚种纯合子(34.29%), 69份出现杂交(65.71%)。结论拉萨市存在较稳定尖音库蚊复合组蚊虫, 蚊密度相对较低, 提示该地区存在蚊虫及蚊媒传染病风险, 应对该地区蚊虫及蚊媒传染病开展监测与风险评估, 为蚊虫及蚊媒传染病控制提供决策依据。

关键词: 蚊虫, 尖音库蚊复合组, 多重PCR技术, 拉萨

Abstract:

Objective To investigate the species composition and population dynamics of mosquitoes in Lhasa, Tibet, China, so as to provide scientific evidence for the surveillance, prevention, and control of mosquitoes and mosquito?borne diseases in this city. Methods Representative research sites were selected based on geographical distributions in Chengguan District in Lhasa. Adult mosquitoes were collected by bed net traps, labor hour method, and light traps in 2009, 2012, and 2013. The trapped mosquitoes were initially identified according to morphological criteria. A proportion of them were examined using a multiplex PCR assay for species confirmation. Results Mosquitoes in Lhasa comprised the subspecies of Culex pipiens complex and Armigeres subalbatus, with subspecies of Cx. pipiens complex as the dominant species. The mean mosquito density monitored by bed net traps was 19.57 mosquitoes per net?hour in 2009 and 3.06 mosquitoes per net?hour in 2012; there was no significant difference in mosquito density between research sites in different years (F1, 1＝0.934, P＞0.05). The mean mosquito density monitored by labor hour method was 62.10 mosquitoes per person?hour in 2009 and 7.90 mosquitoes per person?hour in 2012; there was no significant difference in mosquito density between research sites in different years (F1, 1＝0.874, P＞0.05). The mean mosquito density monitored by light traps was 1.47 mosquitoes per trap?hour in 2012 and 0.20 mosquitoes per trap?hour in 2013; there was no significant difference in mosquito density between research sites in different years (F1, 3＝1.503, P＞0.05). A hundred and five mosquitoes captured in 2012 were identified by multiplex PCR, of which 36 (34.29%) were homozygous subspecies of Cx. pipiens complex, while 69 (65.71%) were hybrids. Conclusion Mosquitoes of Cx. pipiens complex subspecies are established in Lhasa, and the population density was relatively low in recent years, indicating that the risk of mosquitoes and mosquito?borne diseases exists in Lhasa. Therefore, surveillance and risk assessment should be conducted to provide a basis for decision?making in the control of mosquitoes and mosquito?borne diseases in this region.

Key words: Mosquito, Culex pipiens complex, Multiplex polymerase chain reaction, Lhasa

中图分类号:

R384.1

刘小波, 次仁顿珠, 郭玉红, 彭措次仁, 白莉, 桑少伟, 白玛次旺, 谷少华, 达珍, 陈斌, 周琳, 万方君, 胡亚萍, 徐俊芳, 王君, 达瓦, 李贵昌, 西绕若登, 刘起勇. 拉萨市2009－2013年蚊虫种类构成及密度动态研究[J]. 中国媒介生物学及控制杂志, 2014, 25(3): 200-204.

LIU Xiao-bo, Cirendunzhu, GUO Yu-hong, Pengcuociren, BAI Li, SANG Shao-wei, Baimaciwang, GU Shao-hua, Dazhen, CHEN Bin, ZHOU Lin, WAN Fang-jun, HU Ya-ping, XU Jun-fang, WANG Jun, Dawa, LI Gui-chang, Xiraoruodeng, LIU Qi-yong. A survey of species composition and population dynamics of mosquitoesin Lhasa, Tibet, China from 2009 to 2013[J]. Chines Journal of Vector Biology and Control, 2014, 25(3): 200-204.

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拉萨市2009－2013年蚊虫种类构成及密度动态研究

A survey of species composition and population dynamics of mosquitoesin Lhasa, Tibet, China from 2009 to 2013

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