关键词: 淡色库蚊, 杀虫剂, 抗药性, 抗性基因

Abstract:

Objective To determine the insecticide resistance of Culex pipiens pallens from different regions of Zhejiang province, China, to analyze the resistance phenotypes and genotypes of Cx. pipiens pallens and their association, and to provide a baseline data for the mosquito control in Zhejiang. Methods The larva dipping method was used to determine the insecticide resistance of Cx. pipiens pallens from four regions of Zhejiang province, and the genotypes were studied by PCR amplification and sequencing. Results The insecticide resistance of Cx. pipiens pallens varied greatly across different regions of Zhejiang, and beta-cypermethrin, deltamethrin, allethrin, DDVP, acetofenate, or propoxur had a median lethal concentration difference of 2.1 to 42.8 times, with the greatest variations in beta-cypermethrin. The Zhoushan strain had a significantly higher pyrethroid resistance than the strains from other regions. The pyrethroid resistance-associated vssc gene of Zhoushan strain had high polymorphism, and some samples had three neighboring fragment deletions (20 bp, 53 bp, and 40 bp) in the intron region of vssc gene. Conclusion The insecticide resistance phenotype varies greatly in Cx. pipiens pallens from different regions of Zhejiang. The kdr gene of Zhoushan strain has complex polymorphism, further study is warranted to determine whether it is the immediate cause of the change in resistance phenotype.

Key words: Culex pipiens pallens, Insecticide, Insecticide resistance, Resistance gene