中国媒介生物学及控制杂志 ›› 2013, Vol. 24 ›› Issue (6): 506-508.DOI: 10.11853/j.issn.1003.4692.2013.06.008

• 论著 • 上一篇    下一篇

褐家鼠携带汉坦病毒特性与其遗传因素关系的分析

韩旭, 魏亚梅, 韩占英, 张艳波, 齐顺祥, 许永刚, 李琦   

  1. 河北省疾病预防控制中心病毒病防治所, 河北 石家庄 050021
  • 收稿日期:2013-07-16 出版日期:2013-12-20 发布日期:2013-12-20
  • 通讯作者: 李琦,Email:liqinew@126.com
  • 作者简介:韩旭(1982-),男,硕士研究生,主管医师,主要从事自然疫源性疾病防治工作。Email:hbcdchanxu@163.com
  • 基金资助:

    河北省自然科学基金(C2007000944)

Analysis of relationship between hantavirus harborage and genetic factors in Rattus norvegicus

HAN Xu, WEI Ya-mei, HAN Zhan-ying, ZHANG Yan-bo, QI Shun-xiang, XU Yong-gang, LI Qi   

  1. Hebei Center for Disease Control and Prevention, Shijiazhuang 050021, Hebei Province, China
  • Received:2013-07-16 Online:2013-12-20 Published:2013-12-20
  • Supported by:

    Supported by the Natural Science Foundation of Hebei Province (No. C2007000944)

摘要:

目的 了解褐家鼠携带汉坦病毒与其遗传因素间的关系。方法 采用微卫星PCR技术对携带和未携带汉坦病毒的褐家鼠基因组DNA进行PCR扩增,根据所选的10个微卫星位点比较同一地区阴性、阳性标本间的差异。结果 复核肾综合征出血热疫源地所捕获的褐家鼠鼠肺标本48份,挑选8份阳性标本及对应地区的阴性标本16份。通过对同一地区携带与未携带汉坦病毒的褐家鼠间微卫星扩增结果比较得出,8个所选微卫星位点未显现出明显差别,其余2个位点不稳定。结论 经过对褐家鼠在10个微卫星位点的比较,未发现携带和未携带汉坦病毒的褐家鼠之间存在明显差别。

关键词: 肾综合征出血热, 汉坦病毒, 微卫星

Abstract:

Objective To establish the relationship between hantavirus harborage and genetic factors in Rattus norvegicus. Methods The genomic DNAs of R. norvegicus carrying hantavirus and those not carrying hantavirus were amplified by microsatellite PCR. The positive and negative samples in the same area were compared in terms of ten microsatellite loci. Results The 48 lung samples from the R. norvegicus captured in the epidemic areas of hemorrhagic fever with renal syndrome were rechecked, and 8 positive samples (carrying hantavirus) and 16 negative samples (not carrying hantavirus) in the same area were selected. There were no significant differences between the positive samples and negative samples at 8 microsatellite foci, and it was not stable at the other two loci. Conclusion There are no significant differences between R. norvegicus carrying hantavirus and those not carrying hantavirus at the 10 microsatellite loci.

Key words: Hemorrhagic fever with renal syndrome, Hantavirus, Microsatellite

中图分类号: