中国媒介生物学及控制杂志 ›› 2013, Vol. 24 ›› Issue (2): 144-146.

• 论著 • 上一篇    下一篇

新疆地区鼠类汉坦病毒核酸检测和基因分型

郭刚1, 徐军1, 黄林2, 盛金良2, 阿不都扎伊尔3   

  1. 1 阿拉山口出入境检验检疫局,新疆,阿拉山口 833418;
    2 石河子大学动物科技学院,新疆石河子 832003;
    3 新疆出入境检验检疫局
  • 收稿日期:2012-11-05 出版日期:2013-04-20 发布日期:2013-04-20
  • 通讯作者: 盛金良
  • 基金资助:
    国家质检总局科研基金(2012IK245)

Nucleic acid detection and genotyping analysis of Hantavirus among rodents in Xinjiang Uyghur Autonomous Region, China

GUO Gang1, XU Jun1, HUANG Lin2, SHENG Jin-liang2, Abudu zayir3   

  1. 1 Alashankou Entry-Exit Inspection and Quarantine Bureau, Alashankou 833418, Xinjiang Uyghur Autonomous Region, China;
    2 Zoonosis Research Lab, College of Animal Science and Technology Shihezi University, Shihezi 832003, Xinjiang Uyghur Autonomous Region, China;
    3 Xinjiang Entry-Exit Inspection and Quarantine Bureau
  • Received:2012-11-05 Online:2013-04-20 Published:2013-04-20
  • Supported by:
    Supported by the Scientific Research Fund Project of Administration of Quality Superision, Inspection and Quarantine(No. 2012IK245)

摘要: 目的 了解新疆地区鼠类中汉坦病毒感染状况及其基因分型.方法 于2010年对新疆3个区域(阿拉山口、石河子、乌鲁木齐)捕获鼠类(沙鼠、田鼠、褐家鼠)采集肺脏提取总RNA,经反转录-聚合酶链反应(RT-PCR)扩增汉坦病毒M基因片段;将扩增片段克隆和测序分析,确定病毒基因型.结果 在312份沙鼠和31份田鼠样本中均未检测到目标基因,在72份褐家鼠样本中,检出阳性样本11份,占15.28%,其基因型均为汉城型.结论 首次发现新疆乌鲁木齐市褐家鼠携带汉城型汉坦病毒,应加强该地区肾综合征出血热病例监测和防治工作.

关键词: 鼠类, 褐家鼠, 汉坦病毒, 核酸检测, 基因分型

Abstract: Objective To investigate the prevalence and genotypes of Hantavirus among the rodents in Xinjiang Uyghur Autonomous Region, China. Methods Total RNA was extracted from the lung of rodents (gerbil, vole, and Rattus norvegicus) captured from 3 areas (Alashankou, Shihezi, and Urumqi) of Xinjiang in 2010, and then the Hantavirus M gene was amplified by RT-PCR. The amplified gene fragments were cloned and then sequenced to identify their genotypes. Results No target fragment was detected in 312 gerbil lung samples and 31 vole lung samples. Of the 72 R. norvegicus lung samples, 11 (15.28%) were positive with Hantavirus M gene, all belonging to SEOV genotype. Conclusion It is the first time to find the Hantavirus (SEOV) carried by R. norvegicus in Urumqi, Xinjiang. More measures should be taken to monitor and control hemorrhagic fever with renal syndrome in this area.

Key words: Rodent, Rattus norvegicus, Hantavirus, Nucleic acid detection, Genotyping analysis

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