中国部分地区赫坎按蚊种团的分子鉴别研究

中国媒介生物学及控制杂志 ›› 2013, Vol. 24 ›› Issue (2): 92-97.

中国部分地区赫坎按蚊种团的分子鉴别研究

林琳¹, 杨曼尼¹, 南春燕^1,2, 伍桐^1,3, 马颖¹, 李翔宇¹, 马雅军¹

1 第二军医大学基础医学部病原生物学教研室,上海 200433;
2 陕西师范大学生命科学学院;
3 第二军医大学研究生管理大队临床1队

收稿日期:2012-11-05 出版日期:2013-04-20 发布日期:2013-04-20
通讯作者: 马雅军
基金资助:
国家科技重大专项课题(2012ZX10004219-010)

Molecular taxonomy of Anopheles hyrcanus group in some regions of China

LIN Lin¹, YANG Man-ni¹, NAN Chun-yan^1,2, WU Tong^1,3, MA Ying¹, LI Xiang-yu¹, MA Ya-jun¹

1 Department of Pathogen Biology, Second Military Medical University, Shanghai 200433, China;
2 College of Life Sciences, Shaanxi Normal University;
3 Clinical Company 1, Graduate Administration Division, Second Military Medical University

Received:2012-11-05 Online:2013-04-20 Published:2013-04-20
Supported by:
Supported by the National Science and Technology Major Project for Infectious Disease in China(No. 2012ZX10004219-010)

摘要/Abstract

摘要： 目的阐明我国各地赫坎按蚊种团成员种的组成.方法依据形态鉴定赫坎按蚊种团成员,种类应用PCR法和分子特征确认,分析的分子特征为rDNA-ITS2和rDNA-28S-D3序列.结果对采自我国12个省20个地点的按蚊样本(n＝1259)进行分子鉴别结果显示,该研究样本中包括赫坎按蚊种团成员种(n＝1237)和其它按蚊3种(n＝22);其中赫坎按蚊种团的成员种为中华按蚊、雷氏按蚊、八代按蚊、贵阳按蚊、筠连按蚊、克莱按蚊、比伦按蚊,以及3个未定名种(LL1、LL2和LL3);中华按蚊在大多数采集点密度最大.结论赫坎按蚊种团非常复杂,综合分析确定其成员种类和分布非常重要.

关键词: 赫坎按蚊种团, 分子鉴别

Abstract: Objective To clarify the members of Anopheles hyrcanus group in some regions of China. Methods The members of An. hyrcanus group were identified by morphology, and the sibling species of An. hyrcanus group were determined by PCR and molecular identification. The molecular identification was based on the analysis of rDNA-ITS2 and rDNA-28S-D3 sequences. Results A total of 1259 Anopheles samples were collected from 20 sites in 12 provinces of China. The molecular identification results showed that 1237 of the samples belonged to An. hyrcanus group, and the remaining 22 belonged to 3 other species of Anopheles. The members of An. hyrcanus group were An. sinensis, An. lesteri, An. yatsushiroensis, An. kweiyangensis, An. junlianensis, An. kleini, An. belenrae, and 3 unidentified species (LL1, LL2, and LL3). The population density of An. sinensis was the highest in most collection sites. Conclusion The An. hyrcanus group is complex. It is important to identify the members of this group and their distribution by comprehensive analysis.

Key words: Anopheles hyrcanus group, Molecular taxonomy

中图分类号:

R384.1

林琳, 杨曼尼, 南春燕, 伍桐, 马颖, 李翔宇, 马雅军. 中国部分地区赫坎按蚊种团的分子鉴别研究[J]. 中国媒介生物学及控制杂志, 2013, 24(2): 92-97.

LIN Lin, YANG Man-ni, NAN Chun-yan, WU Tong, MA Ying, LI Xiang-yu, MA Ya-jun. Molecular taxonomy of Anopheles hyrcanus group in some regions of China[J]. Chines Journal of Vector Biology and Control, 2013, 24(2): 92-97.

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