关键词: 蚊虫, 乙型脑炎病毒, 序列分析

Abstract: Objective To investigate the status of mosquitoes carrying Japanese encephalitis (JE) virus in some parts of Tianjin, providing the basis for the control and prevention of JE. Methods The light trap method was used to collect mosquitoes twice a month from July 2010 to September 2010 in Tianjin. Reverse transcription-polymerase chain reaction (RT-PCR) detection was done and the amplified target fragments were sequenced. The evolutionary tree was built with the Mega 4.0 software by the O-linked method. Results A total of 10 285 adult mosquitoes, which belonged to 2 generaes and 3 species (Culex pipiens pallens, Cx. tritaeniorhynchus, and Anopheles sinensis) were captured. Molecular identification showed that the Cx. pipiens pallens was infected with JE viruses, identified by genotyping as JE virus typeⅠand named TJ01 (HQ718463). Conclusion There exists mosquito infection with JE virus typeⅠin Tianjin, which is close to the JE viruses isolated in Shanghai in terms of their evolution.

Key words: Mosquito, Japanese encephalitis virus, Sequence analysis