关键词: 尘螨, 汽车, 孳生, 变应原

Abstract: Objective To investigate the breeding density and the major allergens of acaroid mites in various motors. Methods Acaroid mites were collected from taxis, private cars and buses, and isolated, counted and identified from May to October in 2010. PCR was performed to detect the allergens Der f 1, Der p 1, Der f 2, Der p 2, with statistical analysis of the results done. Results It was found that there were thirteen species of acaroid mites, belonging to 12 genera and 4 families. The breeding rate was the highest in taxis (85.0%) with most species found in private cars. Four gene sequences (646 bp, 923 bp, 455 bp, 408 bp) were specifically amplified from different samples, with the positive rates of Der p 1 and Der p 2 of the samples from taxis higher than those from other motors (80.0% and 65.0%), and the positive rates of the four allergens ranged from 45.0%-57.5% in private cars but much lower in buses. Conclusion The breeding rate, species distribution and positive rates of acaroid mites from the three habitats are significantly different, which may be associated with the variation of temperature and humidity, habits of acaroid mites and degree of human disturbance.

Key words: Acaroid mites, Motors, Breeding, Allergen