关键词: 家蝇, DNA提取, 方法

Abstract: Objective The house fly Musca domestica (Diptera: Muscidae), is a very important disease vector. Research in genetics and molecular biology on this pest is helpful for developing an effective control strategy. In genetic studies, it often needs to use the DNA sample and polymerase chain reaction (PCR) technique. A simple and non-lethal method for isolation of genomic DNA should facilitate studies in population genetics and the generation of fly lines of interest. Methods Genomic DNA was isolated from immersing the hind legs or wings of house flies in the lysis buffer and incubating at 37 ℃ for 1 h. Results Genomic DNA was extracted successfully from wings or legs of individual flies. A fragment of the CYP6D1v1 gene was amplified with the DNA as template. Wing or leg ablation did not affect the survival and fertility of flies. Conclusion The method is fast, simple, effective and non-lethal. The DNA prepared by this method can be used directly in PCR for subsequent analysis such as genotyping. Flies without hind legs or wings can produce enough offspring to establish a population.

Key words: Musca domestica, DNA extraction, Method