中国媒介生物学及控制杂志 ›› 2012, Vol. 23 ›› Issue (1): 39-41.

• 论著 • 上一篇    下一篇

德国小蠊肠道菌BGI-17的鉴定及抗真菌研究

张凡   

  1. 山东师范大学生命科学学院,山东济南 250014
  • 收稿日期:2011-07-21 出版日期:2012-02-20 发布日期:2012-02-20
  • 作者简介:张凡(1979-),男,博士,讲师,从事媒介生物防治工作。Email: brandzhang@tom.com
  • 基金资助:

    山东省教育厅科技发展计划项目(J11LC13,J07YG02);山东省中青年科学家科研奖励基金资助项目(2007BS02014)

Identification and anti-fungal activity of intestinal bacterium BGI-17 from Blattella germanica

ZHANG Fan   

  1. Shandong Normal University, Ji'nan 250014, Shandong Province, China
  • Received:2011-07-21 Online:2012-02-20 Published:2012-02-20
  • Supported by:

    Supported by the Project of Shandong Province Higher Educational Science and Technology Program(No. J11LC13,J07YG02) and the Scientific Research Foundation for the Young Scientists of Shandong Province(No. 2007BS02014)

摘要:

目的 对分离自德国小蠊肠道的细菌BGI-17进行鉴定,并研究其对昆虫致病真菌的抑制效果,为探明德国小蠊肠道菌群的组成及其与虫体间的关系打下基础。方法 利用PCR扩增该菌16S rDNA序列,根据测序结果构建系统发育树,结合形态及生理生化特征对BGI-17进行鉴定;利用平板抑制法测定该菌对白僵菌的抑菌效果,并观察该菌对真菌菌丝生长的影响。结果 该菌短杆状,周生鞭毛,革兰染色、芽孢染色、接触酶为阳性,初步鉴定为芽孢杆菌属,基于16SrDNA基因序列构建的进化树显示,该菌与萎缩芽孢杆菌相似度达到99%,两者所构建的系统发育树处于同一个分支;该菌对白僵菌生长有明显抑制效果,真菌菌丝出现病态的空泡状膨大,导致原生质外泄和菌丝断裂。结论 德国小蠊肠道菌BGI-17属萎缩芽孢杆菌,可拮抗昆虫致病真菌,可能有助于德国小蠊抵御病原真菌的侵染。

关键词: 德国小蠊, 肠道细菌, 16S rDNA, 鉴定, 萎缩芽孢杆菌, 抗真菌

Abstract:

Objective To identify an intestinal bacterium BGI-17 isolated from German cockroaches (Blattella germanica) and to study its activity of anti-pathogenic fungi so as to clarify the composition and relationship of the intestinal flora in B. germanica. Methods The strain BGI-17 was identified according to the morphology and physiological and biochemical characteristics, and the phylogenetic tree was generated based on the sequence of 16S rDNA for molecular identification. The test for anti-Beauveria bassiana was conducted using the plate inhibition method and the growth of fungi was observed. Results The short, rod-shaped BGI-17 was positive for flagellum, Gram stain, spore stain and catalase. The 16S rDNA sequencing of BGI-17 showed that it shared 99% similarity with the published sequence of Bacillus atrophaeus strain, and both sequences constituted a branch in phylogenetic tree. The BGI-17 inhibited the growth of B. bassiana obviously and the fungal mycelium showed sick vacuolar enlargement, leading to leakage and mycelia protoplast rupture. Conclusion The BGI-17 was B.atrophaeus and it showed strong anti-insect pathogenic fungal activity that might contribute to withstanding the infection of pathogenic fungi.

Key words: Blattella germanica, Intestinal flora, 16S rDNA, Identification, Bacillus atrophaeus, Anti-fungal activity

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