中国媒介生物学及控制杂志 ›› 2011, Vol. 22 ›› Issue (5): 427-428,435.

• 论著 • 上一篇    下一篇

2005-2009年河南省肠出血性大肠埃希菌O157∶H7监测分析

李孟磊1, 宋信兵2, 赵嘉咏1, 黄丽莉1, 罗绮3, 尤爱国1, 夏胜利1   

  1. 1. 河南省疾病预防控制中心传染病所,郑州 450016;
    2. 睢县疾病预防控制中心;
    3. 范县疾病预防控制中心
  • 收稿日期:2011-04-16 出版日期:2011-10-20 发布日期:2011-10-20
  • 通讯作者: 夏胜利,Email: xiasl@hncdc.com.cn
  • 作者简介:李孟磊(1980-),男,主管医师,从事传染病预防控制研究。Email: hnscdb@163.com
  • 基金资助:

    国家科技重大专项课题(2009ZX10004-203)

Analysis of enterohemorrhagic Escherichia coli O157∶H7 surveillance data in Henan province, 2005 to 2009

LI Meng-lei1, SONG Xin-bing2, ZHAO Jia-yong1, HUANG Li-li1, LUO Qi3, YOU Ai-guo1, XIA Sheng-li1   

  1. 1. Henan Center for Disease Control and Prevention, Zhengzhou 450016, Henan Province, China;
    2. Suixian Center for Disease Control and Prevention;
    3. Fanxian Center for Disease Control and Prevention
  • Received:2011-04-16 Online:2011-10-20 Published:2011-10-20
  • Supported by:

    Supported by the Major National Science and Technology Projects of China(No. 2009ZX10004-203)

摘要:

目的 监测河南省肠出血性大肠埃希菌O157∶H7在腹泻患者和宿主动物中带菌及毒力基因情况,探索疾病发生的原因和相关因素。方法 对2005-2009年河南省监测点所送可疑菌株进行血清学和PCR复核,对确认菌株进行stx1、stx2、eaeA、hlyA 毒力基因测定。结果 河南省2005-2009年共监测各类标本10 732份,检出O157∶H7菌株255株,检出率为2.38%;其中动物粪便标本的检出率为6.31%,检出率最高的为羊粪(8.04%),其次为牛粪(7.20%);各年份菌株检出率之间有一定差异;河南省肠出血性大肠埃希菌O157∶H7的产毒株主要来自于羊、牛、鸡粪便及蝇类和生肉标本;毒株类型主要为stx2、eaeA、hlyA 组合型。结论 河南省肠出血性大肠埃希菌O157∶H7在人群和各类动物中均存在,最重要的动物宿主是羊和牛;部分食品在加工环节有可能被污染,存在引起暴发的危险性。

关键词: 肠出血性大肠埃希菌O157∶H7, 腹泻, 监测, 动物宿主, 基因型

Abstract:

Objective To determine the presence and virulence genes of enterohemorrhagic Escherichia coli(EHEC) O157∶H7 in diarrhea patients and host animals in Henan province in order to identify the cause and related risk factors. Methods Suspicious samples collected at surveillance sites from 2005 to 2009 were subjected to serological detection and PCR, and tested for stx1, stx2, eaeA, hlyA virulence genes. Results Of the 10 732 serum samples, 255 (2.38%) were positive for O157∶H7 strains. The detection rate in animal stool samples was 6.31% (8.04% for sheep and 7.20% for cows). Different detection rates were observed in different years. Most EHEC O157∶H7 strains were from sheep, cattle and chicken feces. A combination of virulence genes stx2, eaeA, hlyA was predominant. Conclusion EHEC O157∶H7 existed in the population and various animals in Henan province. The most important animal hosts were sheep and cows. Contaminated food during processing was a possible cause and might lead to potential outbreaks.

Key words: Enterohemorrhagic Escherichia coli O157∶H7, Diarrhea, Surveillance, Host animals, Genotype

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