中国媒介生物学及控制杂志 ›› 2011, Vol. 22 ›› Issue (4): 341-343,347.

• 论著 • 上一篇    下一篇

苦楝叶杀螺效果及机制初步研究

李晨光1, 陈钧1, 韩邦兴1,2, 房伟伟1, 杨学娟1   

  1. 1 江苏大学药学院,江苏镇江 212013;
    2 植物细胞工程安徽省工程技术研究中心
  • 收稿日期:2010-12-27 出版日期:2011-08-20 发布日期:2011-08-20

Molluscicidal effect and mechanism of Melia azedarach L. leaves against Oncomelania hupensis

LI Chen-guang1, CHEN Jun1, HAN Bang-xing1,2, FANG Wei-wei1, YANG Xue-juan1   

  1. 1 School of Pharmacy, Jiangsu University, Zhenjiang 212013, Jiangsu Province, China;
    2 Engineering Technology Research Center of Plant Cell Engineering of Anhui Province
  • Received:2010-12-27 Online:2011-08-20 Published:2011-08-20

摘要:

目的 研究苦楝叶石油醚提取物杀螺活性及对湖北钉螺软体组织糖原和蛋白质的生理生化影响,并进行鱼毒的测定。方法 采用系统分离法分离苦楝叶不同极性部位,按照WHO“杀螺剂实验室终筛法”中的浸杀法筛选苦楝叶杀螺活性部位;采用蒽酮显色法、考马斯亮蓝法分别测定苦楝叶杀螺活性部位对钉螺软体组织糖原和蛋白质含量的影响;用斑马鱼急性毒性试验考察其对非靶生物的毒性。结果 苦楝叶杀螺活性部位集中于石油醚提取物,经72 h处理的LC50为101.6 mg/L,并能显著降低钉螺软体组织糖原的含量,减少量为75.61%,且对斑马鱼毒性很小,72 h的LC50为285.6 mg/L。结论 苦楝叶中可能含有安全有效的植物杀螺成分。

关键词: 苦楝叶, 钉螺, 杀螺作用, 糖原, 蛋白质

Abstract:

Objective To identify the molluscicidal effect and mechanism of Melia azedarach L. leaves against Oncomelania hupensis. Methods Different polar parts of M. azedarach L. leaves were isolated to screen for active compounds. The molluscicidal effects of M. azedarach L. leaves at different concentrations were detected using the laboratory screening Methods recommended by WHO. The glycogen and protein contents of O. hupensis treated with light petroleum extract of M. azedarach L. leaves were determined by Anthrone and Coomassie reactions, respectively. Acute toxicity on non-target organisms was determined using Zebrafish as a bioindicator. Results The light petroleum extract of M. azedarach L. leaves was the most active component with snail-killing effects. The LC50 values of the light petroleum extract was 101.6 mg/L after 72 h treatment. The snail glycogen content decreased remarkably (by 75.61%) after treatment with the light petroleum extract. The LC50 value of the zebrafish extract was as small as 285.6 mg/L after 72 h treatment. Conclusion M. azedarach L. leaves may contain safe and effective molluscicidal ingredients.

Key words: Melia azedarach L., Oncomelania snails, Molluscicidal effect, Glycogen, Protein

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