中国媒介生物学及控制杂志 ›› 2011, Vol. 22 ›› Issue (3): 209-211.

• 论著 • 上一篇    下一篇

试验用白纹伊蚊室内标准养殖技术研究

辛正1, 朱春雨2, 李梅3, 王永明1, 王东1, 刘慧媛1, 张洪杰4, 蔡松武5   

  1. 1 济南市疾病预防控制中心病媒生物防制所,济南 250021;
    2 农业部农药检定所;
    3 中国科学院动物研究所;
    4 军事医学科学院微生物流行病研究所;
    5 广东省疾病预防控制中心
  • 收稿日期:2010-12-15 出版日期:2011-06-20 发布日期:2011-06-20
  • 作者简介:辛正(1963-),男,副主任医师,主要从事媒介生物防治工作。Email: xinzheng121@163.com
  • 基金资助:

    2010年农业部农业行业标准制定和修订项目(农财发[2010]49号)

Standard rearing technique of Aedes albopictus in the laboratory

XIN Zheng1, ZHU Chun-yu2, LI Mei3, WANG Yong-ming1, WANG Dong1, LIU Hui-yuan1, ZHANG Hong-jie4, CAI Song-wu5   

  1. 1 Ji'nan Center for Disease Control and Prevention, Ji'nan 250021, Shandong Province, China;
    2 Institute for the Control of Agrochemicals, Ministry of Agriculture;
    3 Institute of Zoology Chinese Academy of Scienc;
    4 Institute of Microbiology and Epidemiology, The Academy of Military Medical Sciences;
    5 Guangdong Center for Disease Control and Prevention
  • Received:2010-12-15 Online:2011-06-20 Published:2011-06-20
  • Supported by:

    Supported by the Agriculture Department of Agriculture-Funded Project to Develop Industry Standards ([2010] No. 49)

摘要:

目的 规范试验用白纹伊蚊的标准化养殖方法,从而获得品质一致的标准化试虫,为驱、灭蚊药物登记试验提供科学、可靠的试验素材,保证登记试验的生物测定效果质量,为科学合理评价驱、灭蚊药物提供技术保证。方法 通过规范白纹伊蚊的养殖条件、饲料质量、饲养技术,以羽化后第3 天雌性成蚊体重为评价指标,验证3批次及多区域养殖技术的可靠性。结果 标准化养殖3批次白纹伊蚊平均体重测定结果表明,3种幼虫养殖配方均可成功养殖出合格的白纹伊蚊,且重复性好,最低质量组相对偏差为8.28%;不同实验室标准化养殖白纹伊蚊平均体重指标测定结果表明,3种配方在不同地区均适用,最低质量组相对偏差为7.69%。结论 使用该养殖技术,不同地区均可成功养殖出供药物效果测定试验用均一体重的白纹伊蚊,且重复性好。

关键词: 白纹伊蚊, 养殖技术, 评价指标

Abstract:

Objective To develop standard laboratory rearing techniques for Aedes albopictus in order to provide consistent, quality, standardized testing insects for use in pesticide experiments. Methods Using standard rearing techniques, the mosquitoes were bred under controlled rearing conditions. The reliability of multiregional rearing was examined based on the weight of female adults 3 days after eclosion from three batches of mosquitoes. Results All three batches resulting from standard rearing techniques yielded qualified Ae. albopictus based on the average weight, with high repeatability and low relative deviation (8.28%) of weights. As shown from the average weights of mosquitoes bred using different dietary formulas, all three approaches were applicable to different regions and the relative deviation was low (7.69%). Conclusion The rearing technique was repeatable and effective in developing weight-uniform Ae. albopictus for pesticide experiments.

Key words: Aedes albopictus, Rearing technique, Evaluation indicator

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