中国媒介生物学及控制杂志 ›› 2011, Vol. 22 ›› Issue (1): 11-13.

• 论著 • 上一篇    下一篇

5株虫生真菌对淡色库蚊幼虫的致病力研究

刘丽娟1, 韦玮2, 张本光1, 代玉华1, 王海防1, 程鹏1, 王怀位1, 公茂庆1   

  1. 1. 山东省寄生虫病防治研究所,济宁 272033;
    2. 济宁市第一人民医院
  • 收稿日期:2010-09-03 出版日期:2011-02-20 发布日期:2011-02-20
  • 通讯作者: 公茂庆, Email: maoqingg@yahoo.com
  • 作者简介:刘丽娟(1981-),女,硕士,助理研究员,主要从事昆虫生态、卫生害虫的生物防治研究。Email: 11lljuan@163.com
  • 基金资助:

    山东省科技发展计划项目(2010GSF10207); 山东省医学科学院科技资助项目(2009-08)

Study on the pathogenicity of five strains of entomopathogenic fungi to Culex pipiens pallens larvae

LIU Li-juan1, WEI Wei2, ZHANG Ben-guang1, DAI Yu-hua1, WANG Hai-fang1, CHENG Peng1, WANG Huai-wei1, GONG Mao-qing1   

  1. 1. Shandong Institute of Parasitic Diseases, Jining 272033, Shandong Province, China;
    2. Jining First Peoples Hospital
  • Received:2010-09-03 Online:2011-02-20 Published:2011-02-20
  • Supported by:

    Supported by the Science and Technology Development Plan Project of Shandong Province (No. 2010GSF10207) and Science Project of Shandong Academy of Medical Sciences (No. 2009-08)

摘要:

目的 测定菌株对淡色库蚊幼虫的致病力,筛选出高致病力菌株,为淡色库蚊的防治提供理论依据。方法 选择生物防治中常用的5株菌株,测定不同浓度的孢子悬浮液对淡色库蚊幼虫的累计死亡率,计算半数致死浓度(LC50)。结果 菌株GIM3.428、GIM3.45、GIM3.431、GIM3.95以及GIM3.436对淡色库蚊幼虫均具有致病性,试验10 d,淡色库蚊幼虫累计死亡率分别为100%、82%、79%、73%和57%,菌株对淡色库蚊幼虫的LC50分别为2.98×102、1.41×103、1.26×104、1.02×104、2.07×105孢子/ml。结论 菌株GIM3.428对淡色库蚊幼虫的致病力最高,可将其应用于淡色库蚊的防治。

关键词: 淡色库蚊, 虫生真菌, 菌株, 致病力

Abstract:

Objective To test the pathogenicity of 5 strains of entomopathogenic fungi to Culex pipiens pallens larvae and select the highly pathogenic strain against Cx. pipiens pallens, providing a scientific basis for the prevention and control of the insect. Methods The five fungal strains that are commonly used in biological control were used for the detection of the cumulative motality rates of Cx. pipiens pallens larvae caused by different concentrations of fungal spore suspension, with the median lethal concentration (LC50) values worked out. Results Evaluated based on the growth rate and sporulation, all of the five strains showed different degrees of pathogenicity against Cx. pipiens pallens larvae. At 10 days, the five strains GIM3.428, GIM3.45,GIM3.431, GIM3.95 and GIM3.436 resulted in a cumulative mortality rate of 100%, 82%, 79%, 73% and 57%, with the LC50 being 2.98×102、1.41×103、1.26×104、1.02×104、2.07×105 conidia/ml, respectively. Conclusion The fungal strain GIM3.428, which is of the highest pathogenicity against Cx. pipiens pallens larvae, can be used for the control of the insect.

Key words: Culex pipiens pallens, Entomopathogenic fungi, Strain, Pathogenicity

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