关键词: 病原分离培养, 莱姆病螺旋体鉴定, 基因分型

Abstract: Objective To understand the gene species of lyme agents and provide basic information for lyme disease prevention and control in Jilin province,we investigated the species and distribution of ticks,isolated bacteria from ticks and identified genomic species of Borrelia burdorferi sensu lato.Methods(1)Ticks were caught with flagging method;(2)BSKⅡculture medium was used to isolate the agent,specific McAbs were used to identify the bacteria;(3)PCR-RFLP method was used to identify the species of spirochetes.Results Ticks collected from 11 areas of Jilin province were classified in to two species,Ixodes persulcatus Schulzeand Haemaphysalis concinna Kock.We got seven spirochetes from I.persulcatus Schulze,six strains were belong to B.garinii,another strain is B.afzelii. Conclusion The main vector of lyme disease in Jilin province maybe is I.persulcatus Schulze.There are at least two gene species,B.garinii and B.afzelii.These two genespecies are pathogenic to people,so we should improve the prevention and control of lyme disease in Jilin province.