中国媒介生物学及控制杂志

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应用凝血反应检测褐家鼠种群抗药性水平

孙毅1;易建荣2;梁练3;吴太平4;郭天宇1   

  1. 1军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室 北京100071;2广东省疾病预防控制中心;3广东省湛江鼠疫防治研究所;4湖北省疾病预防控制中心
  • 出版日期:2006-02-20 发布日期:2006-02-20

Anticoagulant Resistance Monitoring with Blood Clotting Response Tests in Rattus norvegicus

SUN Yi; YI Jian-rong; LIANG Lian; WU Tai-ping; GUO Tian-yu   

  1. State Key Laboratory of Biosecurity and Pathogen, Institute of Microbiology and Epidemiology of Beijing, Beijing 100071, China
  • Online:2006-02-20 Published:2006-02-20

摘要: 目的 测定褐家鼠的抗药性水平,比较凝血反应与摄食实验测定结果的差异。方法 采用凝血反应法和摄食法分别测定褐家鼠对杀鼠灵和溴敌隆的抗药性。结果 褐家鼠对杀鼠灵ED50为3.849(雄)和4.727 mg/kg(雌),对溴敌隆ED50为0.516(雄)和0.689 mg/kg(雌)。按照鼠药抗性委员会(ARRPC)推荐的敏感褐家鼠基准,该褐家鼠种群对杀鼠灵的抗性水平在2.219~2.549之间,对溴敌隆的抗性水平在1.098~1.129之间。摄食实验也获得了类似结果。结论 凝血反应是一种简便、可行的褐家鼠抗药性测试方法。

关键词: 凝血反应, 褐家鼠, 抗药性, 杀鼠灵, 溴敌隆

Abstract: Objective Evaluation the resistance of Rattus norvegicus against warfarin and bromadiolone and comparison the two testing methods,blood clotting response(BCR) and feeding.Methods The resistance of R.norvegicus against warfarin and bromadiolone was tested with BCR and feeding method.Results The 50% effective dosees(ED50) of warfarin for R.norvigicus Beijing suburb population were(3.849 mg/kg) and(4.727 mg/kg) for male and female respectively.As for bromadiolone,ED50 values were(0.516 mg/kg) and(0.689 mg/kg) for male and female.According to the baseline of susceptibleR.norvegicus approved by ARRPC,the resistance of the population was(2.219-2.549) fold for warfarin and(1.098-1.129) for bromadiolone.Conclusion Compared with feeding test approved by WHO in 1980s,BCR methods obtained similar results and appeared to be a reliable and applic20050822able method in monitor anticoagulant resistance of commensal rodent.