中国媒介生物学及控制杂志

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云南三带喙库蚊实验种群的建立及生物学特性观察

冯云1;张海林1;亚红祥1;张云智1;杨卫红1;章域震1;米竹青1;龚正达1;俞永新2;董关木2   

  1. 1云南省地方病防治所病毒立克次体研究中心 大理671000;2中国药品生物制品检定所
  • 出版日期:2004-12-20 发布日期:2004-12-20

Establishment Laboratorial Population of Yunnan Strain of Culex tritaeriorhynchus and Their Biological Characters

FENG Yun;ZHANG Hai-lin;YA Hong-xiang;et al   

  1. Yunnan Institute of Endemic Diseases Control and Prevention,Dali 671000,China
  • Online:2004-12-20 Published:2004-12-20

摘要: 目的建立三带喙库蚊实验室种群,观察其生物学特性。方法采用人工控制温湿度和光照、模拟自然环境的方法,幼虫饲料为牛肝馒头粉,成蚊供血动物为兔子和小白鼠。结果在室温24~27℃,昼夜温差<2℃,相对湿度70%~80%及人工光照12~14h的条件下养殖,并在蚊笼中放入枝叶较嫩、多汁、无毒的植物,成蚊从叮吸兔血逐渐过渡到叮吸小白鼠血。于2003年8月至2004年1月成功繁殖了7个世代。它们的平均孵化率、蛹化率和羽化率依次为75.64%、80.74%、90.97%,经9~16d可完成一个世代的繁殖。羽化后的成蚊1~2d开始交配,雌蚊第3天开始吸血,第6天达吸血高峰。吸血后第3天开始产卵。结论驯化繁殖三带喙库蚊成功的关键在于提高成活率、交配率、吸血率,所获数据对进一步了解该蚊的生长繁殖特性及防治提供了依据。

关键词: 三带喙库蚊, 实验室种群, 生物学特性

Abstract: Objective To obtain a laboratory population of Culex tritaeriorhynchus,and evaluate their biological characters.Methods Larvae were reared using powder of ox liver and steamed bum( 1∶1) and adults were fed by bloods of rabbit and mouse,under the conditions of temperature between 24 ℃ and 27 ℃(less than 2 ℃ difference for daytime and night),the relative humidity was from 70% to 80% with green plants in cages.Results 7 generations had been propagated successfully from August 2003 to January 2004.The average hatching rate,pupating rate and eclosion rate was 75.64%,80.74% and 90.97% respectively.The life cycle last from 9 to 16 days.Adults began to mate in the first or the second days after pupating.Females began to bite animals in the third day,biting animals came to peak in the sixth day and began to lay eggs in the third day after biting animals.Conclusion It is the key to increase the survival rate,the mating rate and the mouseblood sucking rate for C.tritaeriorhynchus.