关键词: 德国小蠊, 乙酰胆碱酯酶, 高效氯氰菊酯, 抗药性

Abstract: Objective To more perfectly understand the operational mechanisms of α-cypermethrin and provide theoretical basis and useful methods by researching dosage effect and time effect between α-cypermethrin and acetylcholinesterase(AChE) activities in Blattella germanica.Methods Quantitative α-cypermethrin at different rate was sprayed based on the method of GB13917.1-GB13917.8-92 for insecticide registered testing indoor effect.The activities of AChE were determined by using Acetylcholinesterase(AChE) Kit.Results Inhibition of α-cypermethrin on AChE activities of Blattella germanica showed concentration gradient effect in 48 hours.The results showed the correlation of concentration of α-cypermethrin and inhibiting time effect on AChE activities of Blattella germanica in 144 hours and death rate of Blattella germanica in 72 hours was positive relationship.Conclusion AChE is one target of α-cypermethrin in Blattella germanica.Because of sensitivity decrease of AChE,target resistance is one of the important resistant mechanisms to α-cypermethrin.The activity of AChE in Blattella germanica may be one of the indices for determining resistant level to pyrethroid insecticides.