中国媒介生物学及控制杂志

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我国蚊虫中昆虫共生微生物Wolbachia感染的研究

宋社吾1;赵彤言1;董言德1;蒋书楠2;陆宝麟1   

  1. 1军事医学科学院微生物流行病研究所 北京100071;2西南农业大学植保系
  • 出版日期:2002-02-20 发布日期:2002-02-20

Study on the Infections of Wolbachia in Mosquitoes in China

SONG She-wu; ZHAO Tong-yan; DONG Yan-de; et al   

  1. Institute of Microbiology Epidemiology, Academy of Military Medical Sciences, Beijing 100071,China
  • Online:2002-02-20 Published:2002-02-20

摘要: 目的:研究我国蚊虫中昆虫共生微生物Wolbachia的感染情况。方法:采用依据Wolbachiawsp基因序列建立的PCR分组检测方法,对我国蚊科中库蚊属、伊蚊属和按蚊属中一些蚊虫种类进行了检测。结果:库蚊属中尖音库蚊复合组4个亚种的实验室种群和伊蚊属中的白纹伊蚊的4个地理株种群均有感染;感染的Wolbachia株属B组中的pip组;同时在白纹伊蚊种群中还发现了有A组和B组Wolbachia的双重感染;而同时检测的三带喙库蚊、刺扰伊蚊、埃及伊蚊的2个地理株、中华按蚊和斯氏按蚊没有发现Wolbachia的感染。结论:本研究应用该方法,成功地对我国蚊虫体内感染的Wolbachia株进行了检测与分组。

关键词: 蚊虫, 聚合酶链反应, Wolbachia

Abstract: Objective:To study the infection of Wolbachia in mosquitoes in China.Method:Specific primers derived from the wsp gene sequence were used to amplify DNA of Wolbachia from mosquitoes in China by polymerase chain reaction(PCR).Results:The characteristic 600bp products were observed after amplification of extracts from 4 subspecies of Culex pipiens complex and 4 geographical strains of Aedes albopictus.However,the products haven't been found in the strains of Aedes aegypti,Aedes vexans, and Anopheles stephensi.The strains of Wolbachia which infect Culex pipiens complex and Ae.albopictus in China belong to pip group by means of the use of group-specific wsp PCR primers.It was found that there were double infections of A and B group Wolbachia in Ae.albopictus in China.Conclusion:The strains of Wolbachiainfected in the mosquitoes in china have been successfully detected and classified by this method.