关键词: 杀虫剂, 家蝇, 飞翔肌线粒体, 荧光偏振, 膜流动性

Abstract: This paper deals with the effects of four pyrethroids and six organophosphates on the fluidity and phase transition temperature of the mitochondrial membrane with fluorescence polarization technique using DPH as a probe. The results showed that the influence of the kind of pyrethroids and organophosphates on membrane fluidity were different. The presence of permethrin or Fenthion、Malathion、Trichlorfon and Omethoate increased the fluidity. But the membrane fluidity were decreased by adding Fenvalerate、Cypermethrin、deltamethrin or phoxim and acephate. Further effect of pyrethroids and organophosphates on the mitochondrial membrane was the shift of the phase transition temperatures. The phase transition temperature decreased by 1℃ in the prescence of Deltamethrin or Acephate、 Omethoate、Fenthion and increased by 2℃、3℃ and 3℃ respectively when adding Cypermethrin or Malathion、 Trichlorfon. Permethrin has no significant effect to phase transition temperature. The results suggest that the effect of pyrethroids and organophosphates on the membrane fluidity may be a critical element in their action mechanisms.