中国媒介生物学及控制杂志

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保幼激素Ⅲ对三带喙库蚊滞育发生期间卵巢滤泡和体内RNA变化的影响

董言德; 薛瑞德; 赵彤言; 姜晓舜; 尤洪涛   

  1. 军事医学科学院微生物流行病研究所 北京100071
  • 出版日期:1998-06-20 发布日期:1998-06-20

Effect of Juvenile Hormone Ⅲ on Ovarian Follicle Development andthe Content of RNA of Culex tritaeniorhynchus Giles in Diapause

Dong Yan-de; Xue Rui-de; Zhao Tong-yan; et al   

  1. Institute of Microbiology & Epidemiology,Beijing 100071
  • Online:1998-06-20 Published:1998-06-20

摘要: 目的:探索保幼激素Ⅲ(JH3)对滞育三带喙库蚊卵巢滤泡和体内RNA变化的影响。方法:短光照和低温联合作用导致三带喙库蚊滞育后,用2ppm 0.5ul或3ppm 0.5ul/蚊外源JH3点滴处理,观察卵巢滤泡发育情况,并测定蚊体内RNA和卵巢内蛋白质含量的变化。结果:低温和短光照引起三带喙库蚊滞育后,卵巢滤泡发育受到抑制,体内RNA的含量明显减少,JH3处理可诱导部分滞育的三带喙库蚊转入发育状态,解除滞育的个体卵巢第一滤泡增大,体内RNA含量增加,但对卵巢蛋白的含量没有影响。结论:JH3可诱导滞育三带喙库蚊卵巢发育,体内RNA合成增加。

关键词: 三带喙库蚊, 滞育, 保幼激素Ⅲ, RNA

Abstract: Ovarian follicle development was retarded and content of RNA decreased after diapause of Culex tritaeniorhynchus Giles occured.The present paper is the results of effect of Juvenile hormone Ⅲ on ovarian follicle development and the content of RNA of Culex tritaeniorhynchus Giles in diapause under experimental condition.It was showed that the smaller follicles in ovaries enlarged and the content of RNA increased after topical treatment with synthetic Juvenile hormone Ⅲ,and it was not found the influence of Juvenile hormone Ⅲ on content of protein in ovarian.