中国媒介生物学及控制杂志 ›› 2018, Vol. 29 ›› Issue (6): 567-570.DOI: 10.11853/j.issn.1003.8280.2018.06.005

• 论著 • 上一篇    下一篇

新疆乌苏市古尔图地区长尾黄鼠感染巴尔通体情况调查

李博1, 岳锡宏1, 卢珊2, 雒涛1, 王信惠1, 黎唯1   

  1. 1 新疆维吾尔自治区疾病预防控制中心鼠疫防治科, 乌鲁木齐 830002;
    2 中国疾病预防控制中心传染病预防控制所, 北京 102206
  • 收稿日期:2018-08-10 出版日期:2018-12-20 发布日期:2018-12-20
  • 通讯作者: 黎唯,Email:cdclw@126.com
  • 作者简介:李博,男,硕士,主管技师,主要从事自然疫源性疾病防控研究,Email:king-2032169@163.com
  • 基金资助:
    中央财政行业专项(131031102000150003)

Analysis on Bartonella infection status of Citellus undulatus in Wusu city Guertu township, Xinjiang

LI Bo1, YUE Xi-hong1, LU Shan2, LUO Tao1, WANG Xin-hui1, LI Wei1   

  1. 1 Xinjiang Center for Disease Control and Prevention, Urumqi 830002, Xinjiang Uygur Autonomous Region, China;
    2 National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention
  • Received:2018-08-10 Online:2018-12-20 Published:2018-12-20
  • Supported by:
    Supported by the Special Funds from the Central Finance (No. 131031102000150003)

摘要: 目的 了解新疆维吾尔自治区(新疆)乌苏市古尔图地区长尾黄鼠的巴尔通体感染状况,为该地区鼠传疾病风险预警与防控措施制定提供科学依据。方法 2017年5-9月采用一日弓形夹法在查岗果勒、布兰布拉克、白石头等地捕获长尾黄鼠86只,无菌采集鼠体肾脏,试剂盒法提取全基因组DNA。应用实时荧光定量PCR法检测样本DNA巴尔通体特异性基因ssrA,计算感染率,分析样本的检测循环阈值(cp值)。结果 42只长尾黄鼠的巴尔通体实时荧光定量PCR检测为阳性,阳性率为48.84%;阳性样本cp值最低为27.49,最高为37.83,其中88.10%阳性样本的cp值在35.00~37.83之间,查岗果勒、布兰布拉克、白石头三地巴尔通体的阳性率分别为48.48%、60.87%和40.00%;布兰布拉克地区巴尔通体感染率、阳性动物染蚤率、蚤指数均高于查岗果勒和白石头地区;三地巴尔通体感染率与动物染蚤率差异无统计学意义(P>0.05)。结论 新疆乌苏市古尔图地区长尾黄鼠间存在巴尔通体自然感染,传播范围广,但群体带菌量较低。

关键词: 巴尔通体, 实时荧光定量PCR, 古尔图地区, 感染调查

Abstract: Objective To understand the infection status of Bartonella in Citellus undulatus from Guertu, and to provide the basis for risk early warning and control measures of rodent-borne diseases. Methods In total, 86 C. undulatus were collected from Chagangguole, Bulanbulake and Baishitou by bow-shaped clip method during 24 hours, the kidneys of the captured rodents were sampled under aseptic condition, genome DNA was extracted and detected by real-time fluorescence quantitative PCR targeting ssrA of Bartonella. The rate of infection were calculated and analyzed by cp test (cp value). Results A total of 86 C. undulatus were tested, the infection rate of Bartonella in those rodents was 48.84% (42/86). The cp of positive samples had the highest value of 37.83 and the lowest value of 27.49, 88.10% of positive samples was in the "35.00-37.83" range. The infection rate of Chagangguole, Bulanlulake and Baishitou were 48.48%, 60.87%, 40.00%, respectively. The flea infection rate and flea index in Bulanlulake were higher than those of other places, the infection rate had no correlation with the flea infection in Guertu (P>0.05). Conclusion There were natural infection of Bartonella among C. undulatus in Guertu, with tendency of wide spread, but pathogen loads were relatively low in wild rodents.

Key words: Bartonella, Real-time fluorescence quantitative PCR, Guertu township, Infection survey

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