中国媒介生物学及控制杂志 ›› 2018, Vol. 29 ›› Issue (1): 50-52.DOI: 10.11853/j.issn.1003.8280.2018.01.013

• 论著 • 上一篇    下一篇

中华按蚊雌成蚊体内海藻糖及海藻糖酶含量变化的研究

刘丽娟1, 胡颖新1, 李亚平2, 程鹏1, 郭秀霞1, 公茂庆1   

  1. 1 山东省医学科学院, 山东省寄生虫病防治研究所, 山东 济宁 272033;
    2 山东省金乡县农业局, 山东 金乡 272200
  • 收稿日期:2017-09-22 出版日期:2018-02-20 发布日期:2018-02-20
  • 作者简介:刘丽娟,女,副研究员,主要从事媒介蚊虫的防制工作,Email:jj8liu@sina.com
  • 基金资助:
    国家自然科学基金(81471985);山东省医学科学院医药卫生科技创新工程

Dynamic changes of trehalose and trehalase in female Anopheles sinensis Wiedemann (Diptera: Culicidae)

LIU Li-juan1, HU Ying-xin1, LI Ya-ping2, CHENG Peng1, GUO Xiu-xia1, GONG Mao-qing1   

  1. 1 Shandong Academy of Medical Sciences, Shandong Institute of Parasitic Diseases, Department of Medical Entomology, Jining 272033, Shandong Province, China;
    2 Jinxiang County Bureau of Agriculture
  • Received:2017-09-22 Online:2018-02-20 Published:2018-02-20
  • Supported by:
    Supported by the National Natural Science Foundation of China (No. 81471985) and the Innovation Project of Shandong Academy of Medical Sciences

摘要: 目的 了解中华按蚊雌成蚊体内海藻糖及海藻糖酶含量的变化规律。方法 利用ELISA测定羽化不同时间的中华按蚊雌成蚊体内的海藻糖及海藻糖酶含量,利用单因素方差和Tukey多重比较方法进行统计学分析。结果 中华按蚊体内海藻糖及海藻糖酶含量随羽化时间的延长先增加后减少,成蚊产卵后,体内海藻糖及海藻糖酶含量低于吸血前。海藻糖含量在羽化第2天达最大值[(2.658 5±0.116 5)mg/g],不同羽化时间海藻糖含量差异有统计学意义(F=21.383,df=100,P<0.05);海藻糖酶在羽化第3天达最大值[(0.111 6±0.002 5)μmol/g]。结论 中华按蚊雌成蚊体内海藻糖及海藻糖酶含量在羽化后均呈规律性变化,可能与蚊虫能量的积累和消耗有关。

关键词: 中华按蚊, 海藻糖, 海藻糖酶, 蚊媒传染病

Abstract: Objective To understand the dynamic changes of trehalose and trehalase of Anopheles sinensis. Methods Enzyme-linked immunosorbent assay (ELISA) was used to detect trehalose and trehalase contents of female adult. ANOVA was used to determine the statistical significance of trehalose and trehalase contents. Subsequently, Tukey's multiple comparison was applied for significance analysis. Results The results showed that the trehalose and trehalase of female An. sinensis increased first and decreased there after post adult emergence. The females after oviposition had lower levels of trehalose and trehalase contents. The contents of trehalose which reached the peak approximately on the second day in adult stage[(2.658 5±0.116 5) mg/g] were significantly different (F=21.383, df=100, P<0.05). However, the contents of trehalase reached the peak stage approximately on the third day in adult stage[(0.111 6±0.002 5) μmol/g]. Conclusion The trehalose and trehalase contents of female An. sinensis have a regular pattern, which is related to the energy accumulation and consumption.

Key words: Anopheles sinensis, Trehalose, Trehalase, Mosquito-borne infectious diseases

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