中国媒介生物学及控制杂志 ›› 2017, Vol. 28 ›› Issue (6): 586-588.DOI: 10.11853/j.issn.1003.8280.2017.06.017

• 调查研究 • 上一篇    下一篇

河北省鼠疫自然疫源地宿主耶尔森菌存在形式初步调查

史献明, 杜国义, 尤殿平   

  1. 河北省鼠疫防治所, 河北 张家口 075000
  • 收稿日期:2017-08-21 出版日期:2017-12-20 发布日期:2017-12-20
  • 通讯作者: 杜国义,Email:dgyhbs@163.com
  • 作者简介:史献明,男,主任医师,从事鼠疫防治工作,Email:wofshi@163.com
  • 基金资助:
    2016年河北省医学重点项目(20170446);国家重点研发计划重点专项(2016YFC1201304)

Study on Yersinia-like bacteria in the plague natural foci of Hebei province

SHI Xian-ming, DU Guo-yi, YOU Dian-ping   

  1. Anti-plague Institute of Hebei Province, Zhangjiakou 075000, Hebei Province, China
  • Received:2017-08-21 Online:2017-12-20 Published:2017-12-20
  • Supported by:
    Supported by the Key Medical Projects of Hebei Province in 2016(No. 20170446)and National Major Research and Development Plan(No. 2016YFC1201304)

摘要: 目的 在鼠疫静息期,检测鼠疫自然疫源地宿主鼠疫耶尔森菌(鼠疫菌)同源菌或变异菌株。方法 2016年5-6月在河北省鼠疫自然疫源地康保牧场监测点,采用夹日法捕获长爪沙鼠,采用弓形夹法捕获达乌尔黄鼠,取各鼠的小肠和舌尖,分离耶尔森菌,进行形态学和生化鉴定、PCR鉴定其种类。结果 在363份标本中,93份为三糖铁琼脂试验阳性菌株,但PCR鉴定排除小肠结肠炎耶尔森菌和假结核耶尔森菌。结论 在鼠疫自然疫源地静息期可能存在鼠疫菌的近缘菌,需要进一步鉴定三糖铁阳性菌株。

关键词: 耶尔森菌同源菌, 鼠疫, 鼠疫自然疫源地

Abstract: Objective To detect the homologous bacteria or mutant strains of Yersinia pestis bubonic plague during the resting period of plague. Methods Meriones unguiculatus were captured by clamping method and Citellus dauricus were captured by bow clamp method in Kangbao rangeland monitoring station of the plague natural foci of Hebei province during May to June in 2016. The intestine and the tip of the tongue were sampled, and Jerson 's bacteria were isolated. Morphological and biochemical identification and PCR identification were carried out. Results In total 93 of 363 samples were found positive for strains of Y. pestis of TSI. But PCR did not validate it. Conclusion There were closely related bacteria to Y. pestis in the natural foci of plague. Further identification of three sugar iron positive strains is needed.

Key words: Yersinia-like bacteria, Plague, The plague natural foci

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