关键词: 鼠疫菌, 遗传学, 四川省

Abstract:

Objective To study the ecotype and molecular characteristics of Yersinia pestis strain that was first isolated from Batang county of Sichuan, China and to provide scientific evidence for developing suitable strategy of plague control and prevention in Batang. Methods Traditional biochemical phenotype identification method and multi-locus variable number tandem repeats analysis (MLVA) were both chosen to identify ecotype and genotype of the tested strain. Traceability analysis was also performed. Results Yersinia pestis strain of Batang was capable of fermenting glycerine, arabinose and maltose, but not rhamnose. Nitrate reduction test of this strain was positive. The MLVA-type of tested strain was the same as Qinghai - Tibet Plateau Ecotype Y. pestis strains isolated from Qinghai province and Tibet Autonomous Region. Conclusion The ecotype of tested strain is Qinghai-Tibet Plateau Ecotype. It has closest relationship with Y. pestis strains distributed in both Qinghai and Tibet. Batang County of Sichuan is a newly discovered natural plague epidemic focus.

Key words: Yersinia pestis, Genetics, Sichuan