广东省湛江市抗溴氰菊酯白纹伊蚊代谢酶活性及其特征研究

中国媒介生物学及控制杂志 ›› 2013, Vol. 24 ›› Issue (2): 103-107.

广东省湛江市抗溴氰菊酯白纹伊蚊代谢酶活性及其特征研究

李袁飒¹, 孟凤霞¹, 蔡松武², 刘起勇¹

1 中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,WHO媒介生物监测与管理合作中心,北京 102206;
2 广东省疾病预防控制中心

收稿日期:2013-01-15 出版日期:2013-04-20 发布日期:2013-04-20
通讯作者: 孟凤霞
基金资助:
国家科技重大专项课题(2008ZX10004-010,2012ZX10004-219)

The resistance of Aedes albopictus adult in Zhanjiang city, Guangdong province to deltamethrin and enzyme activity and its characteristics

LI Yuan-sa¹, MENG Feng-xia¹, CAI Song-wu², LIU Qi-yong¹

1 State Key Laboratory for Infectious Diseases Prevention and Control, WHO Collaborating Centre for Vector Surveillance and Management, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
2 Guangdong Center for Disease Control and Prevention

Received:2013-01-15 Online:2013-04-20 Published:2013-04-20
Supported by:
Supported by the National Science and Technology Major Projects of China(No. 2008ZX10004-010, 2012ZX10004-219)

摘要/Abstract

摘要： 目的了解广东省湛江市白纹伊蚊野外种群对溴氰菊酯抗性水平和多功能氧化酶(MFO)、非特异性酯酶(NSE)、谷胱甘肽转移酶(GSTs)的活力水平及动态特征,为该地区白纹伊蚊控制及抗药性治理提供依据.方法以实验室品系为参考,采用WHO推荐的成蚊接触筒法,测定白纹伊蚊对溴氰菊酯的抗药性,同时用酶标仪测定酶活力;SPSS 20.0软件分析比较种群间酶活力水平、酶活力的频率分布、变异性、酶活力与蛋白质含量的关系等.结果广东省湛江市白纹伊蚊已经对溴氰菊酯产生高抗性;其成蚊的MFO、NSE和GST酶活力分别为实验室参考品系的1.67、1.91和1.96倍,且两种群间3种代谢酶差异有统计学意义(P ＜ 0.05).该野外种群3种代谢酶的活力分布均呈正偏态分布,GST抗性为主要机制,抗性个体高达72.73%,其次为NSE,抗性个体占59.76%,另外还存在2种或3种酶活力同时增高的抗性个体.结论白纹伊蚊湛江种群成蚊对溴氰菊酯的高抗性,可能与3种代谢酶尤其是GST活力增强有关;应加强该地区白纹伊蚊溴氰菊酯等拟除虫菊酯类杀虫剂的抗性监测与机制研究工作,制定切实可行的抗性治理策略.

关键词: 白纹伊蚊, 溴菊菊酯, 多功能氧化酶, 非特异性酯酶, 谷胱甘肽转移酶, 抗药性

Abstract: Objective Objective This study was carried out to know deltamethrin resistant level in Aedes albopictus wild population in Zhanjiang, Guangdong province, to know the enzyme activity of mixed function oxidase(MFO), non-specific esterase(NSE)and glutathione-S-transferases(GSTs), so as to provide evidence for deltamethrin resistant management and for dengue vector control. Methods Using Ae. albopictus laboratory(lab) strains as reference and the WHO recommended adult mosquito pesticide resistance test bioassay the resistance level was evaluated, meanwhile,enzymes’activity was tested by microplate reader. Collected data was analyzed by SPSS 20.0 software to compare the enzymes’activity and the variation between the field and laboratory populations, frequency distribution of enzyme activity, and the relationship between each enzyme’s activity and protein content of mosquito individual. Results Ae. albopictus had developed high resistant to the deltamethrin in Zhanjiang area. Compared with lab strain, the enzyme activity ratio of MFO, NSE, GST in the wild population was 1.67-, 1.91- and 1.96- folds respectively, and there was significant difference between two populations(P ＜ 0.05) on the three tested metabolic enzymes. The distribution of enzyme activity in Zhanjiang population showed a positively skewed distribution. The reshowed that GST was the main metabolic enzyme contributed to the resistantce, with 72.73% resistant individuals, followed by NSE with 59.76% resistance individuals. There were also some individuals had developed resistance to two or three metabolic enzymes in Zhanjiang population. Conclusion Ae. albopictus had developed high resistance to deltamethrin,its resistance mechanisms might be related to MFO,NSE and GST,especially to GST. More attention should be paid to Ae. albopictus pesticide resistance surveillance to make a suitable strategy for pesticide resistance management.

Key words: Aedes albopictus, Deltamethrin, Microsomal mixed function oxidases, Nonspecific esterase, Gultathione-S-transferases, Insecticide resistance

中图分类号:

李袁飒, 孟凤霞, 蔡松武, 刘起勇. 广东省湛江市抗溴氰菊酯白纹伊蚊代谢酶活性及其特征研究[J]. 中国媒介生物学及控制杂志, 2013, 24(2): 103-107.

LI Yuan-sa, MENG Feng-xia, CAI Song-wu, LIU Qi-yong. The resistance of Aedes albopictus adult in Zhanjiang city, Guangdong province to deltamethrin and enzyme activity and its characteristics[J]. Chines Journal of Vector Biology and Control, 2013, 24(2): 103-107.

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