关键词: 汉滩型病毒, 基因亚型, 系统发生分析, 分布

Abstract: Objective To investigate the genetic features and distribution of Hantaan virus (HTNV) in Liaoning province, China. Methods Specimens from mouse lungs and patients with hemorrhagic fever with renal syndrome (HFRS) were collected in the high-epidemic areas of HFRS in Liaoning. The Hantavirus antigens in the mouse lung specimens were detected by indirect immunofluorescence assay. The specific nucleotide sequences of M and S segments in the specimens were amplified by reverse- transcription polymerase chain reaction (RT-PCR). The positive PCR products of HTNV were subject to sequencing and then to homology analysis and phylogenetic analysis. Results The HTNV was carried mainly by Apodemus agrarius in the epidemic areas of HFRS in Liaoning. Four M segments were amplified from the mouse lung specimens, and five M segments and one S segment were amplified from the patient specimens. The homology analysis of the nucleotides of M segments showed that Liaoning strains shared the highest homology (95%-97%) with Bao14 and CJAp267, and had a homology of 87.4%-89.0% with HTNV prototype strain 76-118. The phylogenetic analysis of the M segments showed that the Liaoning strains, along with Bao14 and CJAp267, were in one branch of the phylogenetic tree, belonging to H4 subtype. The homology analysis of the nucleotides of S segment showed that TL2 demonstrated the highest nucleotide homology (97.1%) with YaluRiver13, and had a homology of 91.2% with HTNV prototype strain 76-118. TL2 had homologies of 93.0%-96.2% with Bao14 and CJAp93 and 74.1%-81.6% with other representative strains, according to the amino acid homology deduction for S segment. The results of the phylogenetic analysis based on the S segment were approximately the same as those based on the M segments, suggesting that TL2, Bao14, and CJAp93 were in one branch of the phylogenetic tree. Conclusion H4 subtype HTNV is the most prevalent Hantavirus in Liaoning province, China, with few other genetic subtypes.

Key words: Hantaan virus, Genetic subtype, Phylogenetic analysis, Distribution