中国媒介生物学及控制杂志 ›› 2012, Vol. 23 ›› Issue (5): 424-427.

• 专题报道——虫媒病毒调查研究 • 上一篇    下一篇

甘肃省河西走廊地区2011年蚊虫种类及虫媒病毒调查研究

查冰1,2, 于德山3, 付士红2, 李国太3, 吕志2, 蒋建祥3, 唐承军2, 贾玉新3, 董茂星3, 赵海3, 梁国栋2   

  1. 1. 大理学院病原与媒介生物研究所普洱分部, 云南普洱 665000;
    2. 中国疾病预防控制中心病毒病预防控制所病毒性脑炎室, 传染病预防控制国家重点实验室, 北京 102206;
    3. 甘肃省疾病预防控制中心
  • 收稿日期:2012-07-27 出版日期:2012-10-20 发布日期:2012-10-20
  • 通讯作者: 梁国栋,Email: gdliang@hotmail.com
  • 基金资助:

    国家重点基础研究发展计划(973计划)(2011CB504702)

Investigation of mosquitoes and arboviruses in Hexi Corridorof Gansu province, China in 2011

ZHA Bing1,2, YU De-shan3, FU Shi-hong2, LI Guo-tai3, LÜ Zhi2, JIANG Jian-xiang3, TANG Cheng-jun2, JIA Yu-xin3, DONG Mao-xing3, ZHAO Hai3, LIANG Guo-dong2   

  1. 1. Institute of Pathogens and Vector of Dali University, Puer Division, Puer 665000, Yunnan Province, China;
    2. State Key Laboratoryfor Infectious Diseases Prevention and Control, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Controland Prevention, Beijing 102206, China;
    3. Gansu Center for Disease Control and Prevention
  • Received:2012-07-27 Online:2012-10-20 Published:2012-10-20
  • Supported by:

    Supported by the Grant from the National Basic Research Progrom of China(973 Program)(No. 2011CB504702)

摘要:

目的 调查甘肃省河西走廊地区蚊虫种类及所携带虫媒病毒分布状况。方法 使用诱蚊灯于18:00至次日06:00进行蚊虫标本采集,分类鉴定后液氮保存并运送至实验室备检;通过组织培养法分离病毒,并对病毒分离物进行血清学和分子生物学鉴定;利用生物信息学技术对新分离病毒的序列进行分析,完成同源性和系统发生分析。结果 2011年8月在甘肃省河西走廊地区7个县(市)共采集到蚊虫标本3属6种24028只,其中刺扰伊蚊最多,占采集总数的32.80%(7880/24028),淡色库蚊占30.26%(7272/24028);通过细胞培养分离到1株可以引起两种细胞(BHK-21和C6/36细胞)病变的病毒分离株(GS11-155);此外在32批蚊虫标本中检测到病毒(库蚊黄病毒、盖塔病毒、辽宁病毒)基因阳性,对其中11批核苷酸序列测定和分析发现,1批为蚊传黄病毒,3批为盖塔病毒,7批为辽宁病毒。结论 甘肃省河西走廊地区优势蚊种为刺扰伊蚊,且蚊虫携带多种虫媒病毒,包括蚊传黄病毒、盖塔病毒和辽宁病毒。

关键词: 蚊虫, 库蚊黄病毒, 辽宁病毒, 盖塔病毒

Abstract:

Objective To investigate the species of mosquitoes and the distribution of arboviruses carried by the mosquitoes in Hexi Corridor of Gansu province, China in 2011. Methods Mosquito samples were collected from 6 p.m. to 6 a.m. in the next day with UV lamps. After being identified, the samples were preserved in liquid nitrogen and then transported to a laboratory for examination. Viruses were isolated from the samples by tissue culture, and the virus isolates were identified by serological and molecular biological methods. Homology and phylogenetic analysis was performed on the nucleotide sequences of isolated viruses by bioinformatics. Results A total of 24 028 mosquitoes, belonging to 6 species and 3 genera, were collected in seven counties or cities along Hexi Corridor in August 2011. Among the collected mosquitoes, Aedes vexans was the most abundant species, accounting for 32.80% (7880/24 028), and Culex pipiens pallens accounted for 30.26% (7272/24 028). One virus strain (GS11-155) that could induce pathological changes of two types of cells (BHK-21 and C6/36 cells) was isolated by cell culture. Genes of Culex flavivirus (CxFV), Liaoning virus (LNV), and Getah virus (GETV) were detected in 32 batches of mosquito samples, and in 11 of the 32 batches, 1 had CxFV, 3 had GETV, and 7 had LNV, according to nucleotide sequencing. Conclusion Ae. vexans was the dominant species of mosquitoes in Hexi Corridor of Gansu province, China. The mosquitoes in Hexi Corridor carry several types of arboviruses, including the CxFV, GETV, and LNV

Key words: Mosquito, Culex flavivirus, Liaoning virus, Getah virus

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