中国媒介生物学及控制杂志 ›› 2012, Vol. 23 ›› Issue (5): 410-412,416.

• 专题报道——虫媒病毒调查研究 • 上一篇    下一篇

云南省西双版纳地区2011年蚊虫及虫媒病毒调查

唐承军1, 付士红1, 张海林2, 范建华3, 杨卫红2, 章域震2, 吕志1, 李园园3, 李鸿斌3, 朱进3, 王宇3, 陶伽伶3, 李丽华4, 白卫荣4, 查冰1, 王丕玉5, 梁国栋1   

  1. 1. 中国疾病预防控制中心病毒病预防控制所, 传染病预防控制国家重点实验室, 北京 102206;
    2. 云南省地方病防治所;
    3. 西双版纳州疾病预防控制中心;
    4. 西双版纳州人民医院;
    5. 云南省寄生虫病防治所
  • 收稿日期:2012-07-19 出版日期:2012-10-20 发布日期:2012-10-20
  • 通讯作者: 梁国栋,Email: gdliang@hotmail.com
  • 基金资助:

    国家自然科学基金(81171635);西双版纳州科技项目(201017)

Investigation of mosquitoes and arboviruses in Xishuangbanna,Yunnan province, China in 2011

TANG Cheng-jun1, FU Shi-hong1, ZHANG Hai-lin2, FAN Jian-hua3, YANG Wei-hong2, ZHANG Yu-zhen2, LV Zhi1, LI Yuan-yuan3, LI Hong-bin3, ZHU Jin3, WANG Yu3, TAO Jia-ling3, LI Li-hua4, BAI Wei-rong4, ZHA Bing1, WANG Pi-yu5, LIANG Guo-dong1   

  1. 1. State Key Laboratory for Infectious Diseases Prevention and Control, Institute for Viral Disease Control and Prevention, ChineseCenter for Disease Control and Prevention, Beijing 102206, China;
    2. Yunnan Institute of Endemic Disease Control and Prevention;
    3 Xishuangbanna Center for Disease Control and Prevention;
    4. Xishuangbanna People’s Hospital;
    5. Yunnan Institute of ParasiticDisease Control and Prevention
  • Received:2012-07-19 Online:2012-10-20 Published:2012-10-20
  • Supported by:

    Supported by the National Natural Science Foundation of China(No. 81171635) and Science and Technology Project ofXishuangbanna(No. 201017)

摘要:

目的 了解云南省西双版纳地区蚊虫媒介的分布特点及当地虫媒病毒情况,为虫媒病毒病防治提供科学依据。方法 在云南省西双版纳州采集蚊虫标本,用细胞培养法分离病毒,并用RT-PCR法检测常见虫媒病毒核酸;在西双版纳州采集发热患者血清及脑脊液标本,并用ELISA法检测常见病毒性脑炎IgM抗体。结果 共采获蚊虫5属29种13337只,其中三带喙库蚊、中华按蚊、带足按蚊分别占蚊虫标本总数的79.98%(10667/13337)、7.95%(1060/13337)和7.38%(984/13337),三带喙库蚊为当地优势蚊种。采用流行性乙型脑炎(乙脑)病毒、版纳病毒、甲病毒属、环状病毒等多种虫媒病毒引物对214批蚊虫标本进行PCR检测,结果均为阴性;采用多种细胞对蚊虫标本进行病毒分离,结果也为阴性。用相关脑炎病毒试剂盒对采集到的52份急性期血清标本及54份脑脊液标本进行ELISA检测,发现乙脑病毒IgM阳性16例,单纯疱疹病毒IgM抗体阳性4例,腮腺炎病毒IgM抗体阳性13例,埃可病毒IgM抗体阳性3例,登革热病毒IgM抗体阳性1例。结论 2011年西双版纳地区采集到的蚊虫标本中未检测到乙脑、版纳及环状病毒等虫媒病毒,但血清学检测结果表明当地发热患者存在乙脑等多种病毒性脑炎感染。

关键词: 蚊虫, 虫媒病毒, 流行性乙型脑炎病毒

Abstract:

Objective To investigate the distribution profiles of mosquitoes and arboviruses in Xishuangbanna,Yunnan province, China, and to provide evidence for prevention and control of arbovirus diseases. Methods Mosquito samples were collected in Xishuangbanna and then used for virus isolation by cell culture. RT-PCR was used to identify arbovirus. Serum and cerebrospinal fluid samples were collected from fever patients, and the common encephalitis virus IgM antibodies were detected by ELISA. Results A total of 13 337 mosquitoes, belonging to 29 species and 5 genera, were collected. The main mosquitoes were Culex tritaeniorhynchus (79.98%, 10 667/13 337), followed by Anopheles sinensis (7.95%, 1060/13 337) and An. peditaeniatus (7.38%, 984/13 337). Several specific primers for Japanese encephalitis virus (JEV), Banna virus, alphavirus, and circovirus were used for PCR detection in 214 batches of mosquitoes, and no virus was isolated from the mosquitoes. Also, no virus was found in isolation using several types of cells. A total of 52 serum samples and 54 cerebrospinal fluid samples were collected from patients in acute stage by kits for encephalitis viruses and then subject to ELISA. There were 16 cases with JEV IgM, 4 cases with Herpes simplex virus IgM, 13 cases with mumps virus IgM, 3 cases with ECHO virus IgM, and 1 case with dengue virus IgM. Conclusion No JEV, BAV, and circovirus were detected from mosquitoes collected in Xishuangbanna,Yunnan province, China in 2011. However, serological results indicated that local fever patients were infected with several viruses such as JEV.

Key words: Mosquito, Arbovirus, Japanese encephalitis virus

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