关键词: 家蝇, 高效氟氯氰菊酯, 羧酸酯酶, 抗药性

Abstract:

Objective To determine the association between carboxylesterase (CarE) and beta - cyfluthrin resistance in Musca domestica in various habitats, and monitor this resistance at an early phase by using carboxylesterase. Methods Biological assessment was conducted using dripping titration and the activity of carboxylesterase was determined by the Asperen method. Results Except for the population in transfer stations, the three populations of M. domestica in residential areas, restaurants and farmer's market presented different resistance levels to beta-cyfluthrin with resistance ratios of 1.84, 15.31, 3.19 and 8.84, respectively. The CarE activities in the three populations were significantly elevated (P＜0.05), while 75%, 54% and 72% of them showed remarkably high activities, respectively. Overlapping susceptible strains were found in the three populations except for that in transfer stations. Conclusion Increased CarE activitiy was associated with beta-cyfluthrin resistance in M. domestica. The distribution of high CarE-activity strains showed that the populations in those areas were hybrid resistant strains, which was consistent with the bioassay Results. Based on this finding, it is advisable to restrict the use of beta-cyfluthrin in residential areas and use other pesticides as an alternative to avoid or defer the occurrence of resistance and apply this agent with caution in other habitats.

Key words: Musca domestica, Beta-cyfluthrin, Carboxylesterase, Insecticide resistance