德国小蠊谷胱甘肽S-转移酶在不同发育阶段活性变化及其在野外种群中的水平

中国媒介生物学及控制杂志 ›› 2011, Vol. 22 ›› Issue (5): 429-432.

德国小蠊谷胱甘肽S-转移酶在不同发育阶段活性变化及其在野外种群中的水平

李秋红^1,2, 钱坤², 周小洁², 曾晓芃², 高希武¹

1. 中国农业大学农学与生物技术学院昆虫系,北京 100094;
2. 北京市疾病预防控制中心消毒与有害生物防治所,北京 100013

收稿日期:2011-06-07 出版日期:2011-10-20 发布日期:2011-10-20
通讯作者: 曾晓芃,Email: zengxp@yahoo.cn
作者简介:李秋红(1984-),女,硕士研究生,主要从事媒介生物学防制研究。Email: bargreen@sina.com

Activity of Glutathione S-transferase in Blattella germanica at different developmental stages and from different field strains

LI Qiu-hong^1,2, QIAN Kun², ZHOU Xiao-jie², ZENG Xiao-peng², GAO Xi-wu¹

1. Department of Entomology, China Agriculture University, Beijing 100094, China;
2. Department of Disinfection and Vector Control, Beijing Center for Disease Control and Prevention, Beijing 100013, China

Received:2011-06-07 Online:2011-10-20 Published:2011-10-20

摘要/Abstract

摘要：

目的研究谷胱甘肽S-转移酶(GSTs)在德国小蠊不同发育阶段(若虫到成虫)与北京地区不同野外种群中的活性水平。方法分别采用分光光度法与药膜法测定德国小蠊GSTs活性与其抗性水平。结果德国小蠊GSTs比活力在若虫期随龄期增加而逐渐升高,在4周龄达到最高；随后逐渐降低,在羽化时酶活力降至最低,在成虫期略有升高。德国小蠊敏感品系成虫GSTs比活力为2.70 nmol/(min·mg),K_m值与V_max分别为2.26 mmol/L和0.33 nmol/(min·mg)；9个区(县) 野外种群GSTs比活力为2.76～8.69 nmol/(min·mg),K_m值和V_max分别为0.65～2.37 mmol/L和0.33～0.54 nmol/(min·mg)。与敏感品系比较,野外种群成虫GSTs比活力和V_max均较高,K_m值较低,且对2种常用杀虫剂表现出不同程度的抗性(对高效氯氰菊酯为1.50～8.37倍；对残杀威为1.04～3.34倍)。结论 GSTs活性随德国小蠊发育阶段不同(若虫到成虫)而变化,并可能参与德国小蠊野外种群的抗性形成。

关键词: 德国小蠊, 谷胱甘肽S-转移酶, 抗药性

Abstract:

Objective To study the activity of Glutathione S-transferase (GSTs) of German cockroaches (Blattella germanica) at different developmental stages (from nymph to adult) and different field strains in Beijing. Methods The specific activity of GSTs and resistance levels of B. germanica were determined by spectrophotometry and contact biossay. Results The specific activity of GSTs increased gradually during the nymph period and reached the highest by week 4, which then decreased to the lowest value during eclosion. The specific activity of GSTs in susceptible strains of B. germanica was 2.70 nmol/(min·mg) with K_m of 2.26 mmol/L and V_max of 0.33 nmol/(min·mg); the specific activity of GSTs in nine field strains ranged from 2.76-8.69 nmol/(min·mg) with K_m of 0.65-2.37 mmol/L and V_max of 0.33-0.54 nmol/(min·mg) V_max. Compared with susceptible strains, field strains had higher specific activity and V_max yet lower K_m values. Field strains also demonstrated different degrees of resistance to two commonly used pesticides (cypermethrin 1.50-8.37 fold; propoxur 1.04-3.34 fold). Conclusion GST activity varied in the developmental stages from nymph to adult, which may be involved in the development of insecticide resistance in the field strains of B. germanica.

Key words: Blattella germanica, Glutathione S-transferase, Insecticide resistance

中图分类号:

R384.9

李秋红, 钱坤, 周小洁, 曾晓芃, 高希武. 德国小蠊谷胱甘肽S-转移酶在不同发育阶段活性变化及其在野外种群中的水平[J]. 中国媒介生物学及控制杂志, 2011, 22(5): 429-432.

LI Qiu-hong, QIAN Kun, ZHOU Xiao-jie, ZENG Xiao-peng, GAO Xi-wu. Activity of Glutathione S-transferase in Blattella germanica at different developmental stages and from different field strains[J]. Chines Journal of Vector Biology and Control, 2011, 22(5): 429-432.

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