上海口岸动物宿主携带汉坦病毒及其体表寄生虫监测

摘要/Abstract

摘要：

目的监测上海口岸汉坦病毒在鼠形动物宿主中的携带情况和相关的体外寄生虫种类。方法 2008－2009年每季度对上海市周边的崇明、金山、乍浦等口岸鼠形动物宿主调查1次。通过鼠笼、鼠夹诱捕鼠类,检测其血液和肺组织标本的汉坦病毒。采用ELISA检测血清HFRS-IgG抗体,IgM捕捉ELISA法(MacELISA)检测HFRS-IgM抗体,直接免疫荧光法检测鼠肺组织肾综合征出血热(HFRS)抗原,并通过RT-PCR检测汉坦病毒基因并分型。结果共捕获鼠形动物232只,其中啮齿目4属4种,食虫目2属2种,还有鸟类3种,体外寄生虫有革螨4属4种,蚤2属2种,吸虱1属1种;用各种检测方法未从血清和肺组织样本中检出汉坦病毒。结论上海口岸鼠形动物样本中未分离到汉坦病毒,所捕获的动物其体外寄生昆虫和螨的数量偏少,大麝鼩为上海口岸优势小动物之一,发现黑线姬鼠体表有耶厉螨淮河亚种。

关键词: 上海口岸, 汉坦病毒, 大麝鼩, 耶厉螨淮河亚种

Abstract:

Objective To monitor rodent carriers of Hantan virus, the pathogen of hemorrhagic fever with renal syndrome (HFRS), and related ectozoan vectors in Shanghai coastal harbors. Methods From 2008 to 2009, quarterly surveys of the rodent carriers were performed in Shanghai coastal harbors of Chongming, Jinshan and Zhapu. The rodents were collected using rat cages and clamps. Blood and lung samples were taken for HFRS viral testingand ectoparasites were collected for laboratory identification. The HFRS-IgG and HFRS-IgM antibodies were detected by ELISA, and MacELISA, respectively. HFRS Hantan virus antigens in lung tissue samples were detected by direct immune florescence technique. Hantan virus genotype was analyzed using RT-PCR. Results A total of 232 rodents and 3 birds were captured, including 4 species of 4 genera of Rodentia and 2 species of 2 genera of Insectivora. None of the samples tested positive for HFRS Hantan virus. Conclusion No Hantan virus positive samples were isolated from the captured rodents in the Shanghai coastal harbors, and only a very small amount of ectoparasites were collected from the animals. Crocidura lasiura was the most common animal in these harbors, and Laelaps jettmari huaihoensis comb.n. collected from Apodemus agrarius was a new record in Shanghai.

Key words: Shanghai costal harbors, Hantan virus, Crocidura lasiura, Laelaps jettmari huaihoensis

中图分类号:

丁鲁民. 上海口岸动物宿主携带汉坦病毒及其体表寄生虫监测[J]. 中国媒介生物学及控制杂志, 2011, 22(4): 395-398.

DING Lu-min. Surveillance of animal carriers of Hantan virus and their ectoparasites at Shanghai costal harbors[J]. Chines Journal of Vector Biology and Control, 2011, 22(4): 395-398.

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