酯酶显色法快速检测尖音库蚊对有机磷的抗性

中国媒介生物学及控制杂志 ›› 2011, Vol. 22 ›› Issue (4): 332-335.

酯酶显色法快速检测尖音库蚊对有机磷的抗性

常海静^1,2, 刘洋洋³, 江红², 乔传令², 卢希平³, 郎明林¹, 崔峰²

1 河北农业大学生命科学学院,河北保定 071001;
2 农业虫害鼠害综合治理研究国家重点实验室,中国科学院动物研究所,北京 100101;
3 山东农业大学植物保护学院,山东泰安 271018

收稿日期:2011-02-25 出版日期:2011-08-20 发布日期:2011-08-20
通讯作者: 崔峰,Email: cuif@ioz.ac.cn; 郎明林,Email: minglinlang@yahoo.com.cn
作者简介:常海静(1985-),女,在读硕士,从事生物化学与分子生物学研究。Email: changhai5693@sina.com;刘洋洋(1987-),女,在读硕士,从事植物-有害生物-天敌相互关系研究。Email: yangyangguai20@163.com
基金资助:
国家科技重大专项课题(2008ZX10004-010);中国科学院动物研究所知识创新工程青年人才领域前沿项目(KSCX3-IOZ-1006)

Rapid detection of organophosphate resistance in Culex pipiens complex by acetylcholinesterase staining

CHANG Hai-jing^1,2, LIU Yang-yang³, JIANG Hong², QIAO Chuan-ling², LU Xi-ping³, LANG Ming-lin¹, CUI Feng²

1 College of Life Sciences, Hebei Agriculture University, Baoding 071001, Hebei Province, China;
2 State Key Laboratory of Integrated Management of Pest Insects & Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;
3 Plant Protection College of Shandong Agricultural, Tai'an 271018, Shandong Province, China

Received:2011-02-25 Online:2011-08-20 Published:2011-08-20
Supported by:
Supported by the Major National Science and Technology Projects of China(No. 2008ZX10004-010)and Innovation Program of the Chinese Academy of Sciences(No. KSCX3-IOZ-1006)

摘要/Abstract

摘要：

目的开发能够快速检测野生尖音库蚊种群对有机磷杀虫剂的抗性水平及靶标抗性的试剂盒。方法根据蚊虫抗药性的生化和分子机制,通过建立不同抗性水平的品系,将经典的羧酸酯酶显色与抗性水平对应起来;根据G119S突变导致的不敏感乙酰胆碱酯酶1对不同浓度残杀威抑制的反应,建立靶标抗性与显色反应的关系。结果制备了反映不同有机磷抗性倍数的羧酸酯酶滤纸显色卡,可以快速、简便的检测野生尖音库蚊种群的有机磷抗性水平;建立了用肉眼就可辨别的乙酰胆碱酯酶显色反应体系,快速检测种群中是否存在靶标抗性及其基因型。结论为我国蚊虫抗性检测和预警工作提供了方便、简洁而有效的工具。

关键词: 有机磷杀虫剂, 抗药性, 快速检测, 羧酸酯酶, 乙酰胆碱酯酶

Abstract:

Objective To develop a rapid detection kit for resistance level and target resistance to organophosphate insecticides in Culex pipiens complex field populations. Methods Strains with different resistance levels were established according to biochemical and molecular resistance mechanisms in mosquitoes. to reflect the correlation between typical acetylcholinesterase (AChE) staining and the resistance staining. The relationship between target resistance and chromogenic reactions was determined according to the inhibitory effects of propoxur at different concentrations on insensitive AChE 1 induced by a G119S mutation. Results A set of coloration cards made from AChE filter paper were developed to describe different organophosphate resistance ratios, which could be used for rapid and simple detection of the resistance level in the field. A chromogenic system was established to visualize the presence of target resistance and the relevant genotypes in the population. Conclusion This study introduces a simple and effective tool for detection and monitoring of mosquito resistance.

Key words: Organophosphate insecticide, Insecticide resistance, Quick detection, Esterase, Acetylcholinesterase

中图分类号:

常海静, 刘洋洋, 江红, 乔传令, 卢希平, 郎明林, 崔峰. 酯酶显色法快速检测尖音库蚊对有机磷的抗性[J]. 中国媒介生物学及控制杂志, 2011, 22(4): 332-335.

CHANG Hai-jing, LIU Yang-yang, JIANG Hong, QIAO Chuan-ling, LU Xi-ping, LANG Ming-lin, CUI Feng. Rapid detection of organophosphate resistance in Culex pipiens complex by acetylcholinesterase staining[J]. Chines Journal of Vector Biology and Control, 2011, 22(4): 332-335.

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