中国媒介生物学及控制杂志 ›› 2011, Vol. 22 ›› Issue (4): 319-321,328.

• 论著 • 上一篇    下一篇

一对可以用于喜马拉雅旱獭性别鉴定的引物筛选

马怀雷, 鲁亮, 刘起勇   

  1. 中国疾病预防控制中心传染病预防控制所媒介生物控制室,传染病预防控制国家重点实验室,北京 102206
  • 收稿日期:2011-02-21 出版日期:2011-08-20 发布日期:2011-08-20
  • 通讯作者: 鲁亮,Email: luliang@icdc.cn
  • 作者简介:马怀雷(1986-),男,在读硕士,从事分子生态学的研究。
  • 基金资助:

    国家科技支撑计划项目(2008BAI56B02)

Screening of primers for sex identification of Marmota himalayana

MA Huai-lei, LU Liang, LIU Qi-yong   

  1. State Key Laboratory for Infectious Diseases Prevention and Control, Department of Vector Biology and Control, National Institute for Communicable Diseases Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
  • Received:2011-02-21 Online:2011-08-20 Published:2011-08-20
  • Supported by:

    Supported by the National Science and Technology Support Project of China(No. 2008BAI56B02)

摘要:

目的 从用于黄鼠性别鉴定的srysmcY引物中筛选出能够用于喜马拉雅旱獭性别鉴定的引物。方法 用1对sry 引物和4条smcY 引物的3对组合对已知性别的雌雄各10只喜马拉雅旱獭基因组DNA进行扩增,同时,用1对微卫星引物来验证反应体系的有效性。结果 在验证了PCR反应有效性的前提下,sry 引物从100~1000 bp间扩增出多个条带,且未发现雄性特异性条带,smcY 引物组合1在雄性旱獭中扩增出100 bp左右的亮带,但是部分雌性样品中在600 bp左右扩增出较弱的条带,组合2未扩增出任何条带,组合3的所有雄性在250 bp处扩增出明亮的条带,雌性个体中未见任何条带。结论 smcY引物组合3可以用于喜马拉雅旱獭性别鉴定。

关键词: 性别鉴定, 喜马拉雅旱獭, sry基因, smcY基因

Abstract:

Objective To select a primer set for sex identification of Marmota himalayana from the sry and smcY primers used in ground squirrels. Methods One pair of sry primers and four smcY primers (used in 3 different combinations) were used to amplify the genomes of 10 M. himalayana of known sexes. One pair of microsatellite primers were used to verify the method. Results The sry primers amplified several bands from 100 bp to 1000 bp, but no male-specific bands were found. Combination 1 of smcY primers amplified a bright band of about 100 bp only in males, but a weak band of about 600 bp was also amplified in some females. Using smcY primer combination 2 yielded no amplified bands. Combination 3 amplified a bright band of about 250 bp in all males, but no band was observed in females. Conclusion The gender selectivity of smcY primer combination 3 means it could be used for sex identification of M. himalayana.

Key words: Sex identification, Marmota himalayana, sry gene, smcY gene

中图分类号: