关键词: 黑箱法, 二氧化碳法, 蚊虫, 监测

Abstract:

Objective To compare mosquito densities using the black box method and the carbon dioxide method and explore the application of the black box approach. Methods The black box method and the carbon dioxide method were used to measure mosquito density and composition at seven locations in two districts of Shanghai. Correlations between species composition, sex ratios and seasonal variation were compared using the two Methods. Results The average mosquito density using the black box method (25.63 mosquitoes/box) was 3.50 times higher than the carbon dioxide method (7.32 mosquitoes/ device), and the average density of female mosquitoes using the black box method (14.65 mosquitoes/box) was 2.35 times higher than the carbon dioxide method (6.23 mosquitoes/device), densities were significantly different. The density indices of the two approaches were not correlated. The species composition recorded by the black box method was: Culex pipiens pallens (94.97%), Aedes albopictus (4.97%), Cx. tritaeriorhynchus (0.03%), and Anopheles sinensis (0.03%). The species composition recorded by the carbon dioxide method was: Cx. pipiens pallens (79.26%), Ae. albopictus (18.27%), Cx. tritaeriorhynchus (2.10%) and An. sinensis (0.37%). The differences in composition were also statistically significant. Conclusion As compared to the carbon dioxide method, the black box method is readily applicable, simple, affordable and can be used in most locations, particularly habitats dominated by Cx. pipiens pallens. The carbon dioxide method can be used for the monitoring of both Cx. pipiens pallens and Ae. albopictus.

Key words: Black box method, Carbon dioxide method, Mosquitoes, Surveillance