浙江省家畜动物无形体分子流行病学调查

中国媒介生物学及控制杂志 ›› 2011, Vol. 22 ›› Issue (3): 226-228,232.

浙江省家畜动物无形体分子流行病学调查

陆群英¹, 张立霞², 孙继民¹, 姜理平¹, 柴程良¹, 郑寿贵³, 顾时平⁴, 葛君华⁵, 王誓闻², 禹惠兰², 张丽娟²

1 浙江省疾病预防控制中心微生物所，杭州 310051;
2 中国疾病预防控制中心传染病预防控制所，北京 102206;
3 金华市金东区疾病预防控制中心;
4 安吉县疾病预防控制中心;
5 天台县疾病预防控制中心

收稿日期:2010-12-29 出版日期:2011-06-20 发布日期:2011-06-20
通讯作者: 张丽娟，Email: zhanglijuan@icdc.cn
作者简介:陆群英（1959-），女，硕士，主任医师，从事传染病疾病控制工作。Email: lqycdc@163.com
基金资助:
国家基础研究项目973计划（2010CB530200，2010CB530206）；重大传染病防治科技专项——细菌性传染病病原谱流行规律与变异研究（2009ZX10004-203）及病原体实验室网络化监测技术研究（2008ZX10004-008）

Molecular epidemiological survey of Anaplasma phagtocytophilium in livestock in Zhejiang province

LU Qun-ying¹, ZHANG Li-xia², SUN Ji-min¹, JIANG Li-ping¹, CHAI Cheng-liang¹, ZHENG Shou-gui³, GU Shi-ping⁴, GE Jun-hua⁵, WANG Shi-wen², YU Hui-lan², ZHANG Li-juan²

1 Zhejiang Center for Disease Control and Prevention， Hangzhou 310051, Zhejiang Province, China;
2 National Institute of Communicable Disease Control and Prevention, China CDC, Beijing 102206, China;
3 Jindong District Center for Disease Control and Prevention, Jinhua;
4 Anji Center for Disease Control and Prevention;
5 Tiantai Center for Disease Control and Prevention

Received:2010-12-29 Online:2011-06-20 Published:2011-06-20
Supported by:
Supported by the National Basic Research Project-973 plan （No. 2010CB530200, 2010CB530206）, Special Project of Key Communicable Viral Hepatitis-Research on Infectious Diseases Surveillance Platform of National Sci-Tech Key Project（No. 2009ZX10004-203） and on Pathogen Laboratory Network Surveillance Technique （No. 2008ZX10004-008）

摘要/Abstract

摘要：

目的　调查浙江省天台、安吉及金华县家畜动物牛及山羊无形体感染状况。方法　以在册户籍为准，采用随机方法抽取调查点每农户5只山羊及5头牛，采集5 ml静脉血分离血清，采用WHO推荐的微量间接免疫荧光方法（mIFA）进行血清IgG抗体检测；动物血球标本用于提取DNA进行无形体16S rRNA基因扩增。PCR产物测序并进行同源分析比较，同时进行遗传变异分析。结果　当地家畜动物存在较高无形体携带率（39.42％），主要分布在天台县；无形体流行株16S rRNA基因存在较大变异性，除发现15种独立核酸变异序列外，至少存在5种优势序列变异株。结论　浙江省家畜动物普遍存在无形体携带状况；进一步开展人群无形体病调查及分子流行病学研究十分必要。

关键词: 无形体, 遗传变异, 家畜动物, 浙江省

Abstract:

Objective　To determine the prevalence of Anaplasma phagocytophilum in goats and oxen in Zhejiang province. Methods　Five goats and five oxen were randomly surveyed in each househould based on registration information. Venous blood samples of 5 ml were collected from each subject, and the separated serum was tested for IgG antibodies against A. phagocytophilum using immunofluorescence assay (mIFA) as recommended by the WHO. The blood cells were used for DNA extraction, which was then amplified for the 16S rRNA genes of A. phagocytophilum. The PCR products were sequenced and analyzed for homology and genetic variation. Results　A high carrying rate (39.42%) was found in the local livestock, particularly in Tiantai county. Considerable variation in the 16S rRNA genes was found in the prevalent strains. In addition to 15 independent variations in the nucleic acid sequences, at least five strains with variation in the dominant sequences were found. Conclusion　Most domestic animals in Zhejiang province carry A. phagocytophilum warranting the survey and molecular epidemiological investigation of this pathogen in humans.

Key words: Anaplasma phagocytophilum, Genetic variation, Domestic animals, Zhejiang province

中图分类号:

R376

陆群英, 张立霞, 孙继民, 姜理平, 柴程良, 郑寿贵, 顾时平, 葛君华, 王誓闻, 禹惠兰, 张丽娟. 浙江省家畜动物无形体分子流行病学调查[J]. 中国媒介生物学及控制杂志, 2011, 22(3): 226-228,232.

LU Qun-ying, ZHANG Li-xia, SUN Ji-min, JIANG Li-ping, CHAI Cheng-liang, ZHENG Shou-gui, GU Shi-ping, GE Jun-hua, WANG Shi-wen, YU Hui-lan, ZHANG Li-juan. Molecular epidemiological survey of Anaplasma phagtocytophilium in livestock in Zhejiang province[J]. Chines Journal of Vector Biology and Control, 2011, 22(3): 226-228,232.

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