中国媒介生物学及控制杂志 ›› 2011, Vol. 22 ›› Issue (2): 128-130.

• 论著 • 上一篇    下一篇

高效氯氰菊酯抗性家蝇过氧化物酶性质变化研究

陈德国1, 霍新北2   

  1. 1 山东师范大学生命科学学院,济南 250014;
    2 山东省疾病预防控制中心,济南250014
  • 收稿日期:2010-09-22 出版日期:2011-04-20 发布日期:2011-04-20
  • 通讯作者: 霍新北,Email: sdvbc@126.com
  • 作者简介:陈德国(1981-),男,在读硕士,主要从事媒介生物学及控制研究工作。
  • 基金资助:

    山东省卫生厅科技发展计划项目(2007HZ089)

Study on the glutathione peroxidase activity in Musca domestica resistant to beta-cypermethrin

CHEN De-guo1, HUO Xin-bei2   

  1. 1 Shandong Normal Univercity, College of Life Sciences Jinan 250014, Shandong Province, China;
    2 Shangdong Center for Disease Control and Prevention, Jinan 250014, Shandong Provinc, China
  • Received:2010-09-22 Online:2011-04-20 Published:2011-04-20
  • Supported by:

    Supported by the Science and Technology Development Plan Project of the Health Department of Shandong Province(No. 2007HZ089)

摘要:

目的 研究家蝇抗药性产生机制,为选择快速准确的监测技术和科学用药提供理论依据。方法 通过对实验室培育的抗高效氯氰菊酯品系、抗性衰减品系及敏感品系家蝇的谷胱甘肽-过氧化物酶(GSH-PX)的活性及SDS-聚丙烯酰胺凝胶电泳分析,观察过氧化物酶对家蝇抗性产生的作用。结果 抗性家蝇GSH-PX的活性明显增高,抗性品系家蝇相对活性达到165.89%,抗性衰减品系活性降低到70.13%。3个家蝇品系的电泳图谱中6组条带有较明显的差别。结论 抗高效氯氰菊酯家蝇的过氧化物酶性质发生改变,对家蝇的抗药性产生可能起比较重要的作用。

关键词: 家蝇, 谷胱甘肽-过氧化物酶, 酶活性, SDS-聚丙烯酰胺凝胶电泳

Abstract:

Objective To get an insight into the resistance mechanisms of Musca domestica to beta-cypermethrin so that a theoretical basis was provided for the selection of a rapid and accurate monitoring technique and for the scientific use of insecticides. Methods The glutathione peroxidase(GSH-PX) activities of three strains of M. domestica cultured in laboratory, the beta-cypermethrin resistant strains, the resistance decay strains and the sensitive strains was detected by SDS-PAGE analysis to evaluate the role of the peroxisome in the development of M. domestica resistance. Results It was found that GSH-PX activity of the resistant M. domestica increased significantly with a relative activity of 165.89% in contrast with the activity of the enzyme that decreased to 70.13% in the resistance decay strains. Significant differences were visualized in 6 bands of the electrophoresis pattern of the 3 strains. Conclusion The change in the peroxidase activity in M. domestica strains resistant to beta-cypermethrin may play an important role in the development of M. domestica resistance.

Key words: Musca domestica, Glutathione?peroxidase, Activity, SDS?PAGE

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