中国媒介生物学及控制杂志 ›› 2010, Vol. 21 ›› Issue (5): 457-459.

• 论著 • 上一篇    下一篇

大肠埃希菌O157∶H7家蝇实验带菌消长的研究

丁华,孙建荣,邱丽华,倪晓平   

  1. 杭州市疾病预防控制中心城乡社区公共卫生管理办公室(浙江杭州310021)
  • 收稿日期:2010-03-12 出版日期:2010-10-20 发布日期:2010-10-20
  • 通讯作者: 倪晓平
  • 作者简介:丁华(1968-),女,副主任医师,主要从事疾病预防控制研究工作。Email: dh.dhua999@163.com

Dynamics of Escherichia coli O157∶H7 in infected Musca domestica

DING Hua, SUN Jian-rong, QIU Li-hua, NI Xiao-ping   

  1. Hangzhou Center for Disease Control and Prevention, Hangzhou 310021, Zhejiang Province, China
  • Received:2010-03-12 Online:2010-10-20 Published:2010-10-20
  • Contact: NI Xiao-ping

摘要:

目的 采用大肠埃希菌O157∶H7-EDL933株,实验感染家蝇,观察家蝇体内外感染和带菌消长情况。方法 点滴法体外定量染菌,剂量为1 μl(菌悬液浓度为8.0×105~1.9×107 cfu/ml),并采用昆虫单虫单管方法饲养观察。结果 家蝇经体外染菌,获得体内外带菌,体外带菌时间中位数为(96.0±15.3)h(95%CI:66.0~126.0 h);体内中位数为(144.0±17.3)h(95%CI:110.2~177.8 h);家蝇体内外带菌消长差异无统计学意义(χ2=0.74,P=0.388),家蝇最长带菌时间至少216 h(9 d)。结论 研究结果提示,家蝇不仅是O157∶H7疾病传播的媒介,更重要的可能是人类感染潜在的贮存宿主。

关键词: 大肠埃希菌O157∶H7, 家蝇, 实验感染

Abstract:

Objective To determine the infection status and dynamics in vivo and ex vivo of Escherichia coli O157∶ H7-EDL933 in the infected Musca domestica. Methods In vitro topical application of strains was employed to quantitatively infect the experimental flies, 1 μl apiece (the concentration of bacterial suspension was 8.0×105~1.9×107 cfu/ml). Each fly was bred in one test tube for observation. Results The M. domestica infected In vitro carried the bacteria both in vivo and ex vivo. The median bacteria-carrying duration ex vivo was (96.0±15.3) h (95%CI: 66.0-126.0 h), and the median bacteria-carrying duration in vivo was (144.0 ±17.3) h (95%CI: 110.2-177.8 h). There was no significant difference between the bacteria-carrying dynamics in vivo and ex vivo ( χ2=0.74,P=0.388). The longest bacteria-carrying duration of M. domestica was at least 216 h (9 d). Conclusion M. domestica was not only the probable vector of E. coli O157∶H7, but also a potential reservoir host for human infection thereof.

Key words: Escherichia coli O157∶H7, Musca domestica, Experimental infection

中图分类号: