中国媒介生物学及控制杂志 ›› 2010, Vol. 21 ›› Issue (3): 211-214.

• 论著 • 上一篇    下一篇

石香薷精油对白纹伊蚊的生物活性研究及其成分分析

陈飞飞1,彭映辉1,曾冬琴1,张云1,秦巧慧1, 黄谊2   

  1. 1 中南林业科技大学生命科学与技术学院(湖南 长沙 410004); 2 湖南省疾病预防控制中心
  • 收稿日期:2009-11-22 出版日期:2010-06-20 发布日期:2010-06-20
  • 通讯作者: 彭映辉,Email: pengyh999@163.com
  • 作者简介:陈飞飞 (1983-),男,硕士研究生,主要从事蚊虫防制及植物源农药研究工作。
  • 基金资助:

    湖南省自然科学基金(08JJ3028); 湖南省教育厅高等学校科学研究重点项目(06A083); 湖南省研究生科研创新项目(CX2009B166)

Composition and bioactivity of the essential oils from Mosla chinensis against Aedes albopictus

CHEN Fei-Fei1, PENG Ying-Hui1, ZENG Dong-Qin1, ZHANG Yun1, QIN Qiao-Hui1, HUANG Yi2   

  1. 1 School of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, Hunan Province, China; 2 Hunan Center for Disease Control and Prevention
  • Received:2009-11-22 Online:2010-06-20 Published:2010-06-20
  • Contact: PENG Ying?hui, Email: pengyh999@163.com
  • Supported by:

    Supported by the Hunan Province Natural Science Foundation of China (No. 08JJ3028); Scientific Research Fund of Hunan Province Education Department (No. 06A083) and Hunan Provincial Innovation Foundation For Postgraduate (No. CX2009B166)

摘要:

目的 测定石香薷(Moslem chinensis Maxim)精油对白纹伊蚊(Aedes albopictus)的生物活性,分析石香薷精油的化学成分,为开发植物源蚊虫控制剂提供研究基础。方法 水蒸汽蒸馏法提取石香薷精油;采用浸液法测定精油对白纹伊蚊4龄期幼虫及蛹的毒杀活性;采用人体局部涂肤法测试其对成蚊的驱避活性;通过气相色谱-质谱联用法分析石香薷精油的化学成分。结果 (1)石香薷精油对4龄期幼虫及蛹的24 h LC50值分别为78.820和122.656 μg/ml;(2)涂抹1.5 mg/cm2的纯精油,对成蚊有(2.330±0.167)h的完全保护时间,6 h后仍然有56%的保护率;(3)从精油中共检出44种化合物,鉴定出39种,占总量的98.67%。主要成分有:对-聚伞花素(23.17%)、香荆芥酚(16.83%)、百里香酚(14.92%)、2-异丙基-4-甲基茴香醚(12.89%)、桉树脑(8.48%)、石竹烯(3.96%)、α-石竹烯(3.16%)和乙酸瑞香酯(2.71%)。结论 石香薷精油对白纹伊蚊幼虫及成蚊均有较强的生物活性,具有从中发现新的灭蚊天然活性化合物和研发成环保型蚊虫控制剂的潜力。

关键词: 石香薷, 精油, 白纹伊蚊, 生物活性, 化学成分

Abstract:

Objective The bioactivity of the essential oils from Mosla chinensis against Aedes albopictus was determined, and the chemical composition of the essential oils was analyzed to provide the basis for development of botanical mosquito control agents. Methods The essential oils were extracted from M. chinensis using steam distillation. The bioactivity of the essential oils against 4th instar Ae. albopictus larvae and pupae was determined using immersion tests, and the repellent activity was examined using human skin tests. The gas chromatography?mass spectrometry was employed to analyze the chemical composition of the essential oils from M. chinensis. Results (1) The 24 h LC50 values for 4th instar Ae. albopictus larvae and pupae were 78.820 μg/ml and 122.656 μg/ml, respectively; (2) application of 1.5 mg/cm2 pure essential oils provided (2.330±0.167) h of complete protection time against adult mosquitoes, and 56% of the protective effects remained beyond 6 h; and (3) a total of 44 compounds were detected from the essential oils and 39 were identified, accounting for 98.67%. The main components included p?cymene (23.17%), 5-isopropyl-2-methylphenol (16.83%), thymol (14.92%), Benzene, 2-methoxy-4-methyl-1-(1-methylethyl) (12.89%), eucalyptol (8.48%), caryophyllene (3.96%), α?caryophyllene (3.16%) and thymol acetate (2.71%). Conclusion The essential oils from M. chinensis had strong bioactivities on both Ae. albopictus larvae and adult mosquitoes. New natural active compounds may be found from these essential oils for development of environment?friendly mosquito control agents.

Key words: Mosla chinensis, Essential oil, Aedes albopictus, Bioactivity, Chemical composition

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